再髓鞘化
葛根素
少突胶质细胞
髓鞘
内生
神经干细胞
小胶质细胞
多发性硬化
生物
祖细胞
神经科学
药理学
干细胞
细胞生物学
医学
免疫学
中枢神经系统
炎症
病理
生物化学
替代医学
作者
Hao Xu,Huiyuan Zhang,Nona Pop,Jennifer Hall,Ibrahim Shazlee,Moritz Wagner‐Tsukamoto,Zhiguo Chen,Yuchun Gu,Chao Zhao,Dan Ma
摘要
Abstract Puerarin, a natural isoflavone, is commonly used as a Chinese herbal medicine for the treatment of various cardiovascular and neurological disorders. It has been found to be neuroprotective via TrK‐PI3K/Akt pathway, which is associated with anti‐inflammatory and antioxidant effects. Myelin damage in diseases such as multiple sclerosis (MS) and ischemia induces activation of endogenous oligodendrocyte progenitor cells (OPC) and subsequent remyelination by newly formed oligodendrocytes. It has been shown that human‐induced pluripotent stem cells (hiPSC)‐derived OPCs promote remyelination when transplanted to the brains of disease models. Here, we ask whether and how puerarin is beneficial to the generation of hiPSC‐derived OPCs and oligodendrocytes, and to the endogenous remyelination in mouse demyelination model. Our results show that puerarin increases the proportion of O4+ pre‐oligodendrocytes differentiated from iPSC‐derived neural stem cells. In vitro, puerarin increases proliferation of rat OPCs and enhances mitochondrial activity. Treatment of puerarin at progenitor stage increases the yielding of differentiated oligodendrocytes. In rat organotypic brain slice culture, puerarin promotes both myelination and remyelination. In vivo, puerarin increases oligodendrocyte repopulation during remyelination in mouse spinal cord following lysolethicin‐induced demyelination. Our findings suggest that puerarin promotes oligodendrocyte lineage progression and myelin repair, with a potential to be developed into therapeutic agent for neurological diseases associated with myelin damage. image
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