化学
分子印迹聚合物
介孔二氧化硅
分子印迹
对苯二酚
儿茶酚
介孔材料
检出限
纳米颗粒
肽
色谱法
固定化酶
组合化学
辣根过氧化物酶
吸附
间苯二酚
酚类
酪氨酸酶
苯酚
有机化学
酶
选择性
化学工程
生物化学
催化作用
工程类
作者
Xingguo Liu,Xue Zhang,Zhendong Fu,Xianhui Cheng,Liping Wang,Lianghai Hu
标识
DOI:10.1016/j.aca.2023.340970
摘要
Immobilized enzymes outperform free enzymes in many properties and are widely used in environmental monitoring, engineering applications, food and medical fields. Based on the developed immobilization techniques, the search for immobilization with wider applicability, lower cost and more stable enzyme properties is of significant importance. In this study, we reported a molecular imprinting strategy for immobilizing peptide mimics of DhHP-6 on mesoporous materials. The DhHP-6 molecularly imprinted polymer (MIP) showed much higher adsorption capacity than raw mesoporous silica toward DhHP-6. The DhHP-6 peptide mimics was immobilized on the surface of mesoporous silica for the fast detection of phenolic compounds, a widely spread pollutant with highly toxic and difficult in degradation. Immobilized enzyme of DhHP-6-MIP exhibited higher peroxidase activity, better stability, and recyclability than free peptide. Notably, DhHP-6-MIP showed excellent linearity for the detection of the two phenols with detection limits of 0.28 μM and 0.25 μM, respectively. In combination with the spectral analysis and PCA method, DhHP-6-MIP provided better discrimination between the six phenolic compounds (phenol, catechol, resorcinol, hydroquinone, 2-chlorophenol, 2, 4-dichlorophenol). Our study showed that immobilization of peptide mimics by the molecular imprinting strategy using mesoporous silica as carriers was a simple and effective approach. The DhHP-6-MIP has great potentiality for the monitoring and degradation of environmental pollutants.
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