外周血单个核细胞
病毒复制
免疫系统
生物
微泡
小RNA
自分泌信号
炎症
免疫学
病毒学
体外
病毒
细胞培养
基因
生物化学
遗传学
作者
Julien Boucher,Alyssa Rousseau,Catherine Boucher,Caroline Subra,Wilfried Wenceslas Bazié,Audrey Hubert,Emma Bourgeault,Abderrahim Benmoussa,Benjamin Goyer,Philippe A. Tessier,Caroline Gilbert
出处
期刊:Cells
[MDPI AG]
日期:2023-01-31
卷期号:12 (3): 466-466
被引量:1
标识
DOI:10.3390/cells12030466
摘要
The hallmark of HIV-1 infection is the rapid dysregulation of immune functions. Recent investigations for biomarkers of such dysregulation in people living with HIV (PLWH) reveal a strong correlation between viral rebound and immune activation with an increased abundance of extracellular vesicles (EVs) enriched with microRNA-155. We propose that the activation of peripheral blood mononuclear cells (PBMCs) leads to an increased miR-155 expression and production of miR-155-rich extracellular vesicles (miR-155-rich EVs), which can exacerbate HIV-1 infection by promoting viral replication. PBMCs were incubated with either HIV-1 (NL4.3Balenv), a TLR-7/8 agonist, or TNF. EVs were harvested from the cell culture supernatant by differential centrifugation, and RT-qPCR quantified miR-155 in cells and derived EVs. The effect of miR-155-rich EVs on replication of HIV-1 in incubated PBMCs was then measured by viral RNA and DNA quantification. HIV-1, TLR7/8 agonist, and TNF each induced the release of miR-155-rich EVs by PBMCs. These miR-155-rich EVs increased viral replication in PBMCs infected in vitro. Infection with HIV-1 and inflammation promote the production of miR-155-rich EVs, enhancing viral replication. Such autocrine loops, therefore, could influence the course of HIV-1 infection by promoting viral replication.
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