癌症研究
细胞毒性T细胞
NK-92
免疫学
脐带血
生物
白血病
白细胞介素21
癌细胞
干细胞
川地34
免疫疗法
白细胞介素12
髓系白血病
癌症
体外
T细胞
细胞生物学
免疫系统
生物化学
遗传学
作者
Khadijeh Dizaji Asl,Ali Rafat,Zeinab Mazloumi,Behnaz Valipour,Ali Akbar Movassaghpour,Mehdi Talebi,Majid Mahdavi,Hamid Tayefi Nasrabadi,Hojjatollah Nozad Charoudeh
标识
DOI:10.1016/j.humimm.2022.10.010
摘要
Acute lymphoid (ALL) and myeloid leukemia (AML) are known to be invasive and highly lethal hematological malignancies. Because current treatments are insufficient and have a variety of side effects, researchers are looking for new and more effective therapeutic methods. Interestingly, ongoing efforts to find the best approach to optimize NK cell anti-leukemia potential shed light on the successful treatment of cancer. Mature KIR+NK cells ability to remove HLA Class-I deficient cells has been exploited in cancer immunotherapy. Here, we generated KIR+NK cells from cord blood stem cells using IL-2 and IL-15 cytokines. Our finding underlined the importance of KIR expression in the cytotoxic function of NK cells. Taken together, this study presented an effective in vitro method for the expansion and differentiation of KIR+NK cells using cytokines without any feeder cells. Furthermore, the presented culture condition could be useful for the generation of mature and pure NK cells from limited numbers of CD34+ cord blood cells and might be used as a novel method to improve the current state of cancer therapy.
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