着丝粒
人类人工染色体
生物
染色体
有丝分裂
DNA
遗传学
重复序列
卫星DNA
DNA测序
异染色质
分子生物学
基因组
染色体分离
基因
作者
John Harrington,Gil Van Bokkelen,Robert W. Mays,Karen M. Gustashaw,Huntington F. Willard
出处
期刊:Nature Genetics
[Springer Nature]
日期:1997-04-01
卷期号:15 (4): 345-355
被引量:641
摘要
We have combined long synthetic arrays of alpha satellite DNA with telomeric DNA and genomic DNA to generate artificial chromosomes in human HT1080 cells. The resulting linear microchromosomes contain exogenous alpha satellite DNA, are mitotically and cytogenetically stable in the absence of selection for up to six months in culture, bind centromere proteins specific for active centromeres, and are estimated to be 6–10 megabases in size, approximately one-fifth to one-tenth the size of endogenous human chromosomes. We conclude that this strategy results in the formation of de novo centromere activity and that the microchromosomes so generated contain all of the sequence elements required for stable mitotic chromosome segregation and maintenance. This first-generation system for the construction of human artificial chromosomes should be suitable for dissecting the sequence requirements of human centromeres, as well as developing constructs useful for therapeutic applications.
科研通智能强力驱动
Strongly Powered by AbleSci AI