Long-term effect of manure and mineral fertilizer application on the distribution of organic nitrogen fractions in soil under a rice–wheat cropping system

Abstract A long-term experiment was used to evaluate the effect of integrated nutrient management on the distribution of soil organic N fractions and their contribution to N nutrition of a rice–wheat system. Continuous application of mineral fertilizers, alone or in combination with organic manures for 7 years, led to a marked increase in total N, hydrolysable N (amino acid-N, amino sugar-N, ammonia-N, hydrolysable unknown-N) and non-hydrolysable N compared with their original status in soil. However, continuous rice–wheat cropping without any fertilization resulted in depletion of total N, hydrolysable N and non-hydrolysable N by 21.3, 23.5 and 15.1% over their initial status in surface soil. The effect of press mud (PM) treatment was more pronounced in increasing total and hydrolysable N compared with farmyard manure (FYM) or green manure (GM) treatment. Incorporation of PM, FYM and GM along with mineral fertilizers increased the total N content by 32.8, 18.3 and 5.1% and that of hydrolysable N by 25.7, 19.6 and 9.5%, respectively, over mineral fertilizer treatment. Among the most important fractions, amino sugar-N, amino acid-N and ammonia-N were found to be most the important fractions contributing to grain yield and nitrogen uptake of rice and wheat crops. Keywords: organic N fractionsrice–wheat systemmanuresfertilizers