纳米孔测序
纳米孔
计算生物学
基因组
DNA测序
计算机科学
顺序装配
利用
基因组学
纳米技术
生物
DNA
转录组
基因
遗传学
材料科学
基因表达
计算机安全
作者
Yunhao Wang,Yue Zhao,Audrey E. Bollas,Yuru Wang,Kin Fai Au
标识
DOI:10.1038/s41587-021-01108-x
摘要
Rapid advances in nanopore technologies for sequencing single long DNA and RNA molecules have led to substantial improvements in accuracy, read length and throughput. These breakthroughs have required extensive development of experimental and bioinformatics methods to fully exploit nanopore long reads for investigations of genomes, transcriptomes, epigenomes and epitranscriptomes. Nanopore sequencing is being applied in genome assembly, full-length transcript detection and base modification detection and in more specialized areas, such as rapid clinical diagnoses and outbreak surveillance. Many opportunities remain for improving data quality and analytical approaches through the development of new nanopores, base-calling methods and experimental protocols tailored to particular applications.
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