运动发酵单胞菌
苹果酸
大肠杆菌
苹果酸酶
生物化学
生物
基因
化学
微生物学
酶
发酵
乙醇燃料
脱氢酶
柠檬酸
作者
Rohit Khandelwal,Preeti Srivastava,V. S. Bisaria
标识
DOI:10.1016/j.jbiotec.2022.04.007
摘要
Malic acid is one of the organic acids which is used in various industries including food and pharmaceuticals. Biotechnological production of malic acid by an efficient microorganism is highly desirable as the process will be eco-friendly and cost-effective. In this study, malic acid synthesis by Zymomonas mobilis was studied by expressing Escherichia coli malic enzyme gene under Pchap, Ptac and Ppdc promoters. The mae+ recombinants were obtained by recombineering-based genomic integration of Pchap-mae, Ptac-mae and Ppdc-mae sequences. The Ppdc promoter showed the highest expression of malic enzyme and the Pchap the lowest. However, cell growth was limited in mae+ recombinant containing Ppdc promoter. The metabolic analysis showed the highest level of malic acid in Ppdc-mae recombinant (2.84 g/L), which was about eight times higher than that in the wild type strain. The study showed that these three promoters can be used to produce organic acids in Z. mobilis.
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