Transcriptome sequencing and metabolome analysis reveal the mechanism of Shuanghua Baihe Tablet in the treatment of oral mucositis

粘膜炎 医学 促炎细胞因子 转录组 代谢组学 药理学 代谢组 毒性 生物信息学 内科学 炎症 生物 代谢物 基因表达 生物化学 基因
作者
Qishun Geng,Ruijuan Liu,Zhibo Shen,Wei Qian,Yuanyuan Zheng,Lanqi Jia,Long-Hao WANG,Lifeng Li,Jun Li,Wenhua Xue
出处
期刊:Chinese Journal of Natural Medicines [Elsevier]
卷期号:19 (12): 930-943 被引量:9
标识
DOI:10.1016/s1875-5364(22)60150-x
摘要

Oral mucositis (OM) caused by cancer therapy is the most common adverse reaction in the radiotherapy of head and neck tumors. In severe cases, it can lead to the interruption of treatment, which affects the control of the disease and the quality of life. Shuanghua Baihe Tablet (SBT) is a traditional Chinese medicine (TCM) formula, which is administerd to treat OM in China. It has been clinically effective for more than 30 years, but the underlying mechanism is not completely understood. With the development of multiple omics, it is possible to explore the mechanism of Chinese herbal compound prescriptions. Based on transcriptomics and metabolomics, we explored the underlying mechanism of SBT in the treatment of OM. An OM model of rats was established by 5-FU induction, and SBT was orally administered at dosages of 0.75 and 3 g·kg-1·d-1. In order to search for SBT targets and related metabolites, the dysregulated genes and metabolites were detected by transcriptomics and metabolomics. Immune related indicators such as interleukin-17 (IL-17) and tumor necrosis factor-α (TNF-α) were detected by ELISA. Treg cell disorders was analyzed by flow cytometry. Our results showed that SBT significantly alleviated the symptoms of OM rats and the inflammatory infiltration of ulcer tissues. After SBT administration, inflammatory related metabolic pathways including linoleic acid metabolism, valine, leucine and isoleucine biosynthesis were significantly altered. Furthermore, the production of proinflammatory factors like IL-17 and TNF-α, were also dramatically reduced after SBT administration. Besides, the infiltration degree of Treg cells in the spleen of OM modeling rats was significantly improved by SBT administration, thus maintaining the immune balance of the body. The current study demonstrates that SBT regulates inoleic acid metabolism, glycerophospholipid metabolism and amino acid metabolism, and inhibits IL-17/TNF signal transduction to restore Treg and Th17 cell homeostasis in OM rats, thereby alleviating chemotherapy-induced OM.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
所所应助香蕉雅香采纳,获得10
刚刚
俭朴的皮卡丘完成签到 ,获得积分10
刚刚
深情安青应助Sitong采纳,获得10
刚刚
阿宝驳回了慕青应助
刚刚
lml520完成签到,获得积分10
刚刚
科研通AI2S应助飞快的三问采纳,获得10
刚刚
隐形曼青应助飞快的三问采纳,获得10
刚刚
智慧爷爷发布了新的文献求助10
1秒前
科研小畅发布了新的文献求助10
1秒前
Julie完成签到,获得积分20
1秒前
Ava应助淡淡的秋寒采纳,获得10
1秒前
科目三应助ZQP采纳,获得10
2秒前
老Mark完成签到,获得积分10
2秒前
3秒前
pangmengxuan完成签到,获得积分10
3秒前
奋斗水香发布了新的文献求助10
3秒前
3秒前
4秒前
小洪包完成签到,获得积分20
4秒前
4秒前
5秒前
5秒前
华仔应助陈豆豆采纳,获得10
5秒前
6秒前
森林木发布了新的文献求助10
7秒前
廾匸完成签到,获得积分10
7秒前
7秒前
7秒前
orixero应助优雅烨伟采纳,获得10
7秒前
孤独靖柏发布了新的文献求助10
7秒前
8秒前
Lucas应助PGH采纳,获得10
9秒前
9秒前
唠叨的晟睿完成签到,获得积分10
9秒前
廾匸发布了新的文献求助10
10秒前
10秒前
super chan发布了新的文献求助10
10秒前
SciGPT应助GTY采纳,获得10
10秒前
酷波er应助哒布6采纳,获得10
11秒前
高分求助中
Sustainability in ’Tides Chemistry 2000
The ACS Guide to Scholarly Communication 2000
Studien zur Ideengeschichte der Gesetzgebung 1000
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Threaded Harmony: A Sustainable Approach to Fashion 810
Pharmacogenomics: Applications to Patient Care, Third Edition 800
Gerard de Lairesse : an artist between stage and studio 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3075498
求助须知:如何正确求助?哪些是违规求助? 2728589
关于积分的说明 7505148
捐赠科研通 2376734
什么是DOI,文献DOI怎么找? 1260264
科研通“疑难数据库(出版商)”最低求助积分说明 610928
版权声明 597149