光解酶
细菌
微生物学
单线态氧
紫外线
化学
铜绿假单胞菌
光化学
过氧化氢酶
生物
氧气
材料科学
生物化学
DNA修复
有机化学
酶
基因
遗传学
光电子学
作者
Shijie Yao,Jianfeng Ye,Jing Xia,Yaru Hu,Xue‐Tao Zhao,Jianhao Xie,Kuangfei Lin,Changzheng Cui
标识
DOI:10.1016/j.jhazmat.2022.129549
摘要
The excessive dissemination of New Delhi metallo-β-lactamase-1 (NDM-1), which mediates resistance to a majority of clinical β-lactam antibiotics, has created a major public health problem worldwide. Herein, a blaNDM-1-carrying (plasmid encoded) super-resistant bacterium, Acinetobacter sp. CS-2, was selected to reveal its mechanisms of inactivation and photoreactivation during UV, chlorination and UV/chlorination disinfection. The inactivated CS-2 underwent a certain photoreactivation after UV and chlorination. The logistic model precisely fitted the data obtained in the photoreactivation experiments by UV treatment, with the estimated kinetic parameters Sm (0.530%−12.071%) and k2 (0.0009–0.0471). The photoreactivation of Acinetobacter sp. CS-2 was observed when treated by chlorination at a dosage of 0.5 mg/L with a survival ratio of 34.04%. UV/chlorination not only resulted in the high-efficiency reduction of CS-2 but also effectively controlled its photoreactivation with a survival ratio of 0%− 0.87%. UV/chlorination showed great advantages in causing the irreversible destruction of bacterial surface structures by making the cell membranes wrinkled and incomplete compared with UV disinfection. The singlet oxygen (1O2) generated during UV/chlorination treatment played a vital role in blaNDM-1 removal. This study proposed new insights into the mechanism of inactivation and the characteristics of photoreactivation for the super-resistant bacteria by UV, chlorination and UV/chlorination.
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