Electrospun nanofibers modified with Ni-MOF for electrochemiluminescent determination of glucose

This work was the first to explore the application of Ni-MOF composite electrospinning nanofibers (Ni-MOF NFs) in ECL system, and a novel glucose ECL sensor based on Au NPs and Ni-MOF NFs was constructed. The Ni-MOF NFs, as a co-reaction promoter and as glucose oxidase carrier, was coated on the Au nanoparticles (Au NPs) modified glassy carbon electrode (GCE) to enhance the performance of ECL. The sensor exhibits obvious ECL characteristics and stability under optimal experimental conditions. And the sensor can be successfully applied to detect glucose in human serum. • Ni-MOF composite electrospinning nanofibers (Ni-MOF NFs) was used for the first time to construct a novel ECL biosensor for glucose measurement. • Ni-MOF NFs can be used to as a carrier to immobilize glucose oxidase on the electrode, but also as co-reaction catalyst together with Au NPs. • Taking advantages of Au NPs and Ni-MOF NFs, the proposed ECL biosensor obtained stable and obvious ECL phenomenon. A novel electrochemiluminescence (ECL) sensor for glucose detection was constructed by covering Au nanoparticles (Au NPs), Ni metal–organic frameworks nanofibers (Ni-MOF NFs) and glucose oxidase (GOD) on the electrode surface, respectively. Ni-MOF NFs prepared by electrostatic spinning can be used as GOD carrier and ECL promoter. Meanwhile, synergistic effect of Ni-MOF NFs and Au NPs could amplify the ECL signal of Ru(bpy) 3 2+ -TPrA system. With the increasing of glucose concentration, ECL signal was also increasing, so a method for determining glucose could be established. The possible mechanism of luminescence was investigated, and the parameters affecting the experimental reaction were also studied. Under the optimal conditions, the constructed ECL sensor can effectively detect glucose in the range of 2.0 × 10 −10 to 2.0 × 10 −5 M, and the detection limit was 6.7 × 10 −11 M. This study demonstrated for the first time that Ni-MOF NFs could serve as ECL accelerator for the facile detection of glucose in human serum.