荧光
化学
检出限
水溶液中的金属离子
G-四倍体
猝灭(荧光)
离子
寡核苷酸
水溶液
光诱导电子转移
鸟嘌呤
金属
微量
荧光素
线性范围
分析化学(期刊)
电子转移
光化学
色谱法
DNA
生物化学
物理化学
有机化学
病理
替代医学
核苷酸
物理
基因
医学
量子力学
作者
Yeli Zhang,Wen‐Hua Chen,Xiaoting Dong,Hui Yin Fan,Xiaohua Wang,Liujiao Bian
标识
DOI:10.1016/j.snb.2018.01.131
摘要
Owing to the high toxicity of lead (II) (Pb2+) and silver (I) ions (Ag+) to aquicolous organisms, it is highly desirable to develop a sensitive method for the simultaneous detection of Pb2+ and Ag+. In this work, a novel and sensitive single-labeled fluorescent oligonucleotide (OND) probe is designed to simultaneously detect based on the specific complexation of Ag+ to cytosines, the special induction capacity of Pb2+ to guanine-rich OND to form G-quadruplex and the inherent quenching ability of G-quadruplex to the hexachloro fluorescein (HEX). The Ag+-induced hairpin-link structure makes HEX labeled at the 5′-termini close to the Pb2+-induced G-quadruplex connected at the 3′-terminin, resulting in a remarkable fluorescence quenching owing to photoinduced electron transfer (PET) process from G-quadruplex to HEX. At 298 K, the apparent associating constant between them is 1.85 × 108 (L/mol). Using this system, we can simultaneously detect the Pb2+ and Ag+ over the linear response range of 1–100 nmol/L with a detection limits of 96 pmol/L and 21 pmol/L, respectively. Through the assay in real samples, it is known that this system could be applied to accurately monitor the trace Pb2+ and Ag+ in aqueous solution.
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