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Characterization of the Human Myocardial Proteome in Inflammatory Dilated Cardiomyopathy by Label-free Quantitative Shotgun Proteomics of Heart Biopsies

蛋白质组 扩张型心肌病 蛋白质组学 心肌病 生物 心力衰竭 鸟枪蛋白质组学 射血分数 转录组 定量蛋白质组学 内科学 病理 医学 基因表达 生物信息学 生物化学 基因
作者
Elke Hammer,Michelle Goritzka,K. Satya Prasad,Katrin Darm,Leif Steil,Karin Klingel,Christiane Trimpert,Lars R. Herda,Marcus Dörr,Heyo K. Kroemer,Reinhard Kandolf,Alexander Staudt,Stephan B. Felix,Uwe Völker
出处
期刊:Journal of Proteome Research [American Chemical Society]
卷期号:10 (5): 2161-2171 被引量:68
标识
DOI:10.1021/pr1008042
摘要

Dilated cardiomyopathy (DCM) is characterized by contractile dysfunction leading to heart failure. The molecular changes in the human heart associated with this disease have so far mostly been addressed at the gene expression level and only a few studies have analyzed global changes in the myocardial proteome. Therefore, our objective was to investigate the changes in the proteome in patients suffering from inflammatory DCM (iDCM) and chronic viral infection by a comprehensive quantitative approach. Comparative proteomic profiling of endomyocardial biopsies (EMB) from 10 patients with iDCM (left ventricular ejection fraction <40%, symptoms of heart failure) as well as 7 controls with normal left ventricular function and histology was performed by label-free proteome analysis (LC−MS/MS). Mass spectrometric data were analyzed with the Rosetta Elucidator software package. The analysis covered a total of 485 proteins. Among the 174 proteins displaying at least a 1.3-fold change in intensity (p < 0.05), major changes were observed for mitochondrial and cytoskeletal proteins, but also metabolic pathways were affected in iDCM compared to controls. In iDCM patients, we observed decreased levels of mitochondrial proteins involved in oxidative phosphorylation and tricarboxylic acid cycle. Furthermore, deregulation of proteins of carbohydrate metabolism, the actin cytoskeleton, and extracellular matrix remodeling was observed. Proteomic observations were confirmed by gene expression data and immunohistochemistry (e.g. collagen I and VI). This study demonstrates that label-free, mass spectrometry-centered approaches can identify disease dependent alterations in the proteome from small tissue samples such as endomyocardial biopsies. Thus, this technique might allow better disease characterization and may be a valuable tool in potential clinical proteomic studies.
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