Characterization of the Human Myocardial Proteome in Inflammatory Dilated Cardiomyopathy by Label-free Quantitative Shotgun Proteomics of Heart Biopsies

蛋白质组 扩张型心肌病 蛋白质组学 心肌病 生物 心力衰竭 鸟枪蛋白质组学 射血分数 转录组 定量蛋白质组学 内科学 病理 医学 基因表达 生物信息学 生物化学 基因
作者
Elke Hammer,Michelle Goritzka,K. Satya Prasad,Katrin Darm,Leif Steil,Karin Klingel,Christiane Trimpert,Lars R. Herda,Marcus Dörr,Heyo K. Kroemer,Reinhard Kandolf,Alexander Staudt,Stephan B. Felix,Uwe Völker
出处
期刊:Journal of Proteome Research [American Chemical Society]
卷期号:10 (5): 2161-2171 被引量:68
标识
DOI:10.1021/pr1008042
摘要

Dilated cardiomyopathy (DCM) is characterized by contractile dysfunction leading to heart failure. The molecular changes in the human heart associated with this disease have so far mostly been addressed at the gene expression level and only a few studies have analyzed global changes in the myocardial proteome. Therefore, our objective was to investigate the changes in the proteome in patients suffering from inflammatory DCM (iDCM) and chronic viral infection by a comprehensive quantitative approach. Comparative proteomic profiling of endomyocardial biopsies (EMB) from 10 patients with iDCM (left ventricular ejection fraction <40%, symptoms of heart failure) as well as 7 controls with normal left ventricular function and histology was performed by label-free proteome analysis (LC−MS/MS). Mass spectrometric data were analyzed with the Rosetta Elucidator software package. The analysis covered a total of 485 proteins. Among the 174 proteins displaying at least a 1.3-fold change in intensity (p < 0.05), major changes were observed for mitochondrial and cytoskeletal proteins, but also metabolic pathways were affected in iDCM compared to controls. In iDCM patients, we observed decreased levels of mitochondrial proteins involved in oxidative phosphorylation and tricarboxylic acid cycle. Furthermore, deregulation of proteins of carbohydrate metabolism, the actin cytoskeleton, and extracellular matrix remodeling was observed. Proteomic observations were confirmed by gene expression data and immunohistochemistry (e.g. collagen I and VI). This study demonstrates that label-free, mass spectrometry-centered approaches can identify disease dependent alterations in the proteome from small tissue samples such as endomyocardial biopsies. Thus, this technique might allow better disease characterization and may be a valuable tool in potential clinical proteomic studies.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
2秒前
3秒前
爱笑晓曼发布了新的文献求助20
6秒前
老大蒂亚戈应助YJ888采纳,获得10
7秒前
JamesPei应助潇湘雪月采纳,获得10
7秒前
bbczj发布了新的文献求助10
9秒前
10秒前
11秒前
南风知我意完成签到,获得积分20
12秒前
段一帆发布了新的文献求助30
14秒前
wangqinlei完成签到 ,获得积分10
14秒前
fenghp发布了新的文献求助10
15秒前
王馨雨发布了新的文献求助10
15秒前
17秒前
CipherSage应助ccalvintan采纳,获得10
18秒前
18秒前
雪天的阳完成签到 ,获得积分10
20秒前
21秒前
22秒前
22秒前
烟花应助ren采纳,获得10
23秒前
讨厌科研发布了新的文献求助10
23秒前
量子星尘发布了新的文献求助10
24秒前
苏卿应助科研通管家采纳,获得30
25秒前
fd163c应助科研通管家采纳,获得10
26秒前
香蕉觅云应助科研通管家采纳,获得10
26秒前
思源应助科研通管家采纳,获得10
26秒前
SYLH应助科研通管家采纳,获得10
26秒前
26秒前
CAOHOU应助科研通管家采纳,获得10
26秒前
爆米花应助科研通管家采纳,获得10
26秒前
SYLH应助科研通管家采纳,获得30
26秒前
小蘑菇应助科研通管家采纳,获得10
26秒前
26秒前
殷勤的紫槐完成签到,获得积分10
26秒前
风轻青柠发布了新的文献求助10
27秒前
27秒前
机智冬灵完成签到,获得积分10
28秒前
29秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989390
求助须知:如何正确求助?哪些是违规求助? 3531487
关于积分的说明 11254109
捐赠科研通 3270153
什么是DOI,文献DOI怎么找? 1804887
邀请新用户注册赠送积分活动 882087
科研通“疑难数据库(出版商)”最低求助积分说明 809174