Tissues-based chemical profiling and semi-quantitative analysis of bioactive components in the root of Salvia miltiorrhiza Bunge by using laser microdissection system combined with UPLC-q-TOF-MS

激光捕获显微切割 丹参 化学 色谱法 仿形(计算机编程) 传统医学 计算生物学 生物化学 中医药 计算机科学 生物 病理 基因表达 医学 基因 替代医学 操作系统
作者
Xie Wenjian,Hongjie Zhang,Jianguo Zeng,Hubiao Chen,Zhongzhen Zhao,Zhitao Liang
出处
期刊:Chemistry Central Journal [Springer Nature]
卷期号:10 (1) 被引量:14
标识
DOI:10.1186/s13065-016-0187-7
摘要

The dry root of Salvia miltiorrhiza Bunge (Danshen in Chinese) is an used-widely traditional Chinese herbal medicine with and promising efficacy. This herbal plant has been extensively cultivated in China. Currently, people usually rely on its morphological features to evalaute its pharmaceutical quality. In this study, laser micro-dissection system (LMD) was applied to isolate single fresh tissue of root of S. miltiorrhiza. Under fluorescent microscopic model, five tissues namely cork, cortex, phloem, xylem ray and vessel were well recognized and isolated accurately by LMD, respectively and then the distribution pattern of the major bioactive compounds in various tissues was investigated by ultra-performance liquid chromatography-quadrupole/time of flight-mass spectrometry, which aims to validate the traditional experience on evaluating pharmaceutical quality of Danshen by morphological features.Total 62 chemical peak signals were captured and 58 compounds including 33 tanshinones, 23 salvianolic acids and 2 others were identified or tentatively characterized in micro-dissection tissues. Further semi-quantitative analysis indicated that the bioactive components such as tanshinones and salvianolic acids were mainly enriched in cork tissue.In the present study, analysis of metabolic profile in different tissues of roots of S. miltiorrhiza is reported for the first time. The distribution pattern of major bioactive components could enable medicinal vendors and consumers to relatively determine the pharmaceutical quality of Danshen by morphological features.Graphical abstractTissues-based chemical profiling of Danshen.
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