亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Route to Rheumatoid Arthritis by Macrophage-Derived Microvesicle-Coated Nanoparticles

体内 化学 微泡 巨噬细胞 体外 微泡 PLGA公司 细胞生物学 炎症 生物化学 生物 免疫学 基因 小RNA 生物技术
作者
Ruixiang Li,Yuwei He,Ying Zhu,Lixian Jiang,Shuya Zhang,Jing Qin,Qian Wu,Wentao Dai,Shun Shen,Zhiqing Pang,Jianxin Wang
出处
期刊:Nano Letters [American Chemical Society]
卷期号:19 (1): 124-134 被引量:271
标识
DOI:10.1021/acs.nanolett.8b03439
摘要

The targeted delivery of therapeutics to sites of rheumatoid arthritis (RA) has been a long-standing challenge. Inspired by the intrinsic inflammation-targeting capacity of macrophages, a macrophage-derived microvesicle (MMV)-coated nanoparticle (MNP) was developed for targeting RA. The MMV was efficiently produced through a novel method. Cytochalasin B (CB) was applied to relax the interaction between the cytoskeleton and membrane of macrophages, thus stimulating MMV secretion. The proteomic profile of the MMV was analyzed by iTRAQ (isobaric tags for relative and absolute quantitation). The MMV membrane proteins were similar to those of macrophages, indicating that the MMV could exhibit bioactivity similar to that of RA-targeting macrophages. A poly(lactic- co-glycolic acid) (PLGA) nanoparticle was subsequently coated with MMV, and the inflammation-mediated targeting capacity of the MNP was evaluated both in vitro and in vivo. The in vitro binding of MNP to inflamed HUVECs was significantly stronger than that of the red blood cell membrane-coated nanoparticle (RNP). Compared with bare NP and RNP, MNP showed a significantly enhanced targeting effect in vivo in a collagen-induced arthritis (CIA) mouse model. The targeting mechanism was subsequently revealed according to the proteomic analysis, indicating that Mac-1 and CD44 contributed to the outstanding targeting effect of the MNP. A model drug, tacrolimus, was encapsulated in MNP (T-RNP) and significantly suppressed the progression of RA in mice. The present study demonstrates MMV as a promising and rich material, with which to mimic macrophages, and demonstrates that MNP is an efficient biomimetic vehicle for RA targeting and treatment.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
虞美人发布了新的文献求助10
4秒前
科研通AI5应助XWY采纳,获得10
8秒前
顾矜应助罗皮特采纳,获得10
10秒前
猫猫逃离二次元完成签到,获得积分10
10秒前
11秒前
15秒前
大意的绿蓉完成签到,获得积分10
15秒前
16秒前
19秒前
尊敬雪萍发布了新的文献求助10
21秒前
英姑应助重要的夏烟采纳,获得10
23秒前
李健应助ssynkl采纳,获得10
23秒前
Leffzeng完成签到,获得积分10
24秒前
demonox发布了新的文献求助10
25秒前
XWY完成签到,获得积分10
26秒前
Ava应助Leffzeng采纳,获得10
30秒前
XWY发布了新的文献求助10
32秒前
qiu发布了新的文献求助10
39秒前
ehsl完成签到,获得积分10
40秒前
李清湛完成签到,获得积分10
49秒前
52秒前
54秒前
Leffzeng发布了新的文献求助10
58秒前
随便发布了新的文献求助10
59秒前
青山完成签到 ,获得积分10
1分钟前
1分钟前
从容成危完成签到,获得积分10
1分钟前
会飞的鱼发布了新的文献求助10
1分钟前
冰冰完成签到,获得积分20
1分钟前
1分钟前
阿司匹林完成签到 ,获得积分10
1分钟前
Ying完成签到,获得积分10
1分钟前
yuwen发布了新的文献求助10
1分钟前
QiongYin_123完成签到 ,获得积分10
1分钟前
苏梨子完成签到,获得积分10
1分钟前
余念安完成签到 ,获得积分10
1分钟前
SciGPT应助科研通管家采纳,获得10
1分钟前
1分钟前
Ava应助科研通管家采纳,获得10
1分钟前
1分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968293
求助须知:如何正确求助?哪些是违规求助? 3513229
关于积分的说明 11166833
捐赠科研通 3248478
什么是DOI,文献DOI怎么找? 1794268
邀请新用户注册赠送积分活动 874956
科研通“疑难数据库(出版商)”最低求助积分说明 804629