亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Quantitative phosphoproteomic analysis of the molecular substrates of sleep need

睡眠(系统调用) 睡眠剥夺 磷酸化 高磷酸化 神经科学 生物 蛋白质组 细胞生物学 昼夜节律 生物信息学 计算机科学 操作系统
作者
Zhiqiang Wang,Jing Ma,Chika Miyoshi,Yuxin Li,Makito Sato,Yukino Ogawa,Tingting Lou,Chengyuan Ma,Xue Gao,Chiyu Lee,Tomoyuki Fujiyama,Xiaojie Yang,Shuang Zhou,Noriko Hotta-Hirashima,Daniela Klewe-Nebenius,Aya Ikkyu,Miyo Kakizaki,Sanae Kanno,Liqin Cao,Satoru Takahashi,Junmin Peng,Yonghao Yu,Hiromasa Funato,Masashi Yanagisawa,Qinghua Liu
出处
期刊:Nature [Springer Nature]
卷期号:558 (7710): 435-439 被引量:196
标识
DOI:10.1038/s41586-018-0218-8
摘要

Sleep and wake have global effects on brain physiology, from molecular changes1-4 and neuronal activities to synaptic plasticity3-7. Sleep-wake homeostasis is maintained by the generation of a sleep need that accumulates during waking and dissipates during sleep8-11. Here we investigate the molecular basis of sleep need using quantitative phosphoproteomic analysis of the sleep-deprived and Sleepy mouse models of increased sleep need. Sleep deprivation induces cumulative phosphorylation of the brain proteome, which dissipates during sleep. Sleepy mice, owing to a gain-of-function mutation in the Sik3 gene 12 , have a constitutively high sleep need despite increased sleep amount. The brain proteome of these mice exhibits hyperphosphorylation, similar to that seen in the brain of sleep-deprived mice. Comparison of the two models identifies 80 mostly synaptic sleep-need-index phosphoproteins (SNIPPs), in which phosphorylation states closely parallel changes of sleep need. SLEEPY, the mutant SIK3 protein, preferentially associates with and phosphorylates SNIPPs. Inhibition of SIK3 activity reduces phosphorylation of SNIPPs and slow wave activity during non-rapid-eye-movement sleep, the best known measurable index of sleep need, in both Sleepy mice and sleep-deprived wild-type mice. Our results suggest that phosphorylation of SNIPPs accumulates and dissipates in relation to sleep need, and therefore SNIPP phosphorylation is a molecular signature of sleep need. Whereas waking encodes memories by potentiating synapses, sleep consolidates memories and restores synaptic homeostasis by globally downscaling excitatory synapses4-6. Thus, the phosphorylation-dephosphorylation cycle of SNIPPs may represent a major regulatory mechanism that underlies both synaptic homeostasis and sleep-wake homeostasis.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
调研昵称发布了新的文献求助10
7秒前
9秒前
无语发布了新的文献求助10
14秒前
19秒前
xpqiu完成签到,获得积分10
28秒前
45秒前
无语完成签到,获得积分20
51秒前
Sooinlee完成签到,获得积分10
59秒前
1分钟前
阿明发布了新的文献求助10
1分钟前
Nancy0818完成签到 ,获得积分10
1分钟前
Tirachen完成签到,获得积分10
1分钟前
Tirachen发布了新的文献求助10
1分钟前
情怀应助后会无期采纳,获得10
2分钟前
2分钟前
动听乐萱发布了新的文献求助10
2分钟前
科目三应助动听乐萱采纳,获得10
2分钟前
2分钟前
后会无期发布了新的文献求助10
3分钟前
wizardz完成签到 ,获得积分10
3分钟前
3分钟前
陌路完成签到,获得积分10
3分钟前
科研搬运工完成签到,获得积分10
3分钟前
陌路发布了新的文献求助10
3分钟前
CATH完成签到 ,获得积分10
3分钟前
3分钟前
4分钟前
zzz2193发布了新的文献求助10
4分钟前
华仔应助后会无期采纳,获得10
4分钟前
城南烤地瓜完成签到 ,获得积分10
4分钟前
4分钟前
5分钟前
iehaoang完成签到 ,获得积分10
5分钟前
5分钟前
zzz完成签到 ,获得积分10
5分钟前
动听乐萱发布了新的文献求助10
5分钟前
Erin完成签到 ,获得积分10
5分钟前
彭于晏应助阿明采纳,获得10
5分钟前
Artin发布了新的文献求助100
5分钟前
香蕉觅云应助科研通管家采纳,获得10
5分钟前
高分求助中
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Gerard de Lairesse : an artist between stage and studio 500
Digging and Dealing in Eighteenth-Century Rome 500
Queer Politics in Times of New Authoritarianisms: Popular Culture in South Asia 500
Livre et militantisme : La Cité éditeur 1958-1967 500
Retention of title in secured transactions law from a creditor's perspective: A comparative analysis of selected (non-)functional approaches 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3062985
求助须知:如何正确求助?哪些是违规求助? 2717920
关于积分的说明 7456576
捐赠科研通 2364178
什么是DOI,文献DOI怎么找? 1253324
科研通“疑难数据库(出版商)”最低求助积分说明 608499
版权声明 596552