单克隆抗体
抗体
免疫分析
生物
病毒学
单克隆
免疫球蛋白G
分子生物学
杂交瘤技术
免疫学
作者
Ruiyin Chu,Jacob Gerstein,Hai Wu,Haibin Huang,Yang Lou,Rachel Palmer
出处
期刊:Monoclonal antibodies in immunodiagnosis and immunotherapy
[Mary Ann Liebert]
日期:2020-10-01
卷期号:39 (5): 175-183
被引量:3
标识
DOI:10.1089/mab.2020.0024
摘要
During preclinical studies, there is a great need to develop monoclonal antibodies (mAbs) that are specific to human immunoglobulin (IgG), without binding to monkey IgG, to detect therapeutic human mAb in non-human primates. We took advantage of the latest rabbit B cell cloning technology to develop six unique rabbit anti-human IgG mAb clones for this purpose. These clones are capable of binding to both human IgG and Fab with high affinity without nonspecific binding to cynomolgus monkey IgG. These clones have been evaluated as a generic capture reagent for the detection of human IgG and Fab, in the presence of cynomolgus monkey serum, by Gyrolab™ immunoassay. They may be used in singlet or as pairs for the detection of human IgG, in any host animal, to meet the need for therapeutic mAb development in preclinical studies.
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