间充质干细胞
罗哌卡因
活力测定
医学
利多卡因
干细胞
布比卡因
局部麻醉剂
药理学
脂肪组织
细胞毒性T细胞
细胞
体外
细胞生物学
病理
麻醉
化学
生物
内分泌学
生物化学
作者
Eva Kubrova,Min Su,Catalina Galeano‐Garces,M. Lizeth Galvan,Sofía Jerez,Allan B. Dietz,Jay Smith,Wenchun Qu,André J. van Wijnen
出处
期刊:American Journal of Physical Medicine & Rehabilitation
[Ovid Technologies (Wolters Kluwer)]
日期:2020-07-10
卷期号:100 (1): 82-91
被引量:10
标识
DOI:10.1097/phm.0000000000001529
摘要
Purpose We evaluated biological effects of distinct local anesthetics on human adipose-derived mesenchymal stem cells when applied to reduce periprocedural pain during mesenchymal stem cell injections. Methods and Materials Metabolic activity (MTS assay), viability (Live/Dead stain), and gene expression (quantitative real-time reverse-transcriptase polymerase chain reaction) were measured in mesenchymal stem cells incubated with various concentrations of lidocaine, ropivacaine, or bupivacaine during a 12-hr time course. Results Cell viability and metabolic activity decreased in a dose, time, and substance-specific manner after exposure to lidocaine, ropivacaine, and bupivacaine, with ropivacaine being the least cytotoxic. Cell viability decreases after brief exposure (<1.5 hrs) at clinically relevant concentrations (eg, 8 mg/ml of lidocaine, 2.5 mg/ml of ropivacaine or bupivacaine). Mesenchymal stem cells exposed to local anesthetics change their expression of mRNA biomarkers for stress response (EGR1, EGR2), proliferation (MKI67, HIST2H4A), ECM (COL1A1, COL3A1), and cell surface marker (CD105). Conclusions Local anesthetics are cytotoxic to clinical-grade human mesenchymal stem cells in a dose-, time-, and agent-dependent manner and change expression of ECM, proliferation, and cell surface markers. Lidocaine and bupivacaine are more cytotoxic than ropivacaine. Single-dose injections of local anesthetics may affect the biological properties of mesenchymal stem cells in vitro but may not affect the effective dose of MSCs in a clinical setting.
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