Pretreatment with Retro‐2 protects cells from death caused by ricin toxin by retaining the capacity of protein synthesis

蓖麻毒素 活力测定 毒素 细胞因子 信使核糖核酸 蛋白质生物合成 内质网 外周血单个核细胞 生物 细胞毒性 细胞培养 促炎细胞因子 细胞 生物化学 细胞生物学 分子生物学 药理学 免疫学 炎症 体外 基因 遗传学
作者
Zhouguang Jiao,Yuehua Ke,Sha Li,Duo Su,Changjiao Gan,Lingfei Hu,Xiaodong Zhao,Bo Gao,Yajun Song,Dongsheng Zhou,Yefeng Qiu,Huiying Yang
出处
期刊:Journal of Applied Toxicology [Wiley]
卷期号:40 (10): 1440-1450 被引量:4
标识
DOI:10.1002/jat.3997
摘要

Abstract The current study explores the detoxification effect of Retro‐2 on ricin toxin (RT) cytotoxicity, as well as the mechanisms underlying such effects, to provide a basis for follow‐up clinical applications of Retro‐2. The mouse‐derived mononuclear/macrophage cell line, RAW264.7, was used to evaluate the detoxification effect of Retro‐2 on RT by detecting cell viability, capacity for protein synthesis and the expression of cytokines, as well as endoplasmic reticulum stress (ERS)‐related mRNA. The results indicated that many cells died when challenged with concentrations of RT ≥50ng/mL. The protein synthesis capacity of cells decreased when challenged with 200ng/mL RT for 2hours. Furthermore, the synthesis and release of many cytokines decreased, while the expression of cytokines or ERS‐related mRNA increased when challenged with 200ng/mL of RT for 12 or more hours. However, cell viability, capacity for protein synthesis and release levels of many cytokines were higher, while the expression levels of cytokine, or ERS‐related mRNA, were lower in cells pretreated with 20μm Retro‐2 and challenged with RT, compared with those that had not been pretreated with Retro‐2. In conclusion, Retro‐2 retained the capacity for protein synthesis inhibited by RT, alleviated ERS induced by RT and increased the viability of cells challenged with RT. Retro‐2 shows the potential for clinical applications.
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