CD4/CD8 + T cells, DC subsets, Foxp3, and IDO expression are predictive indictors of gastric cancer prognosis

FOXP3型 CD8型 肿瘤浸润淋巴细胞 调节性T细胞 流式细胞术 癌症 肿瘤微环境 医学 癌症研究 免疫组织化学 免疫系统 T细胞 免疫学 白细胞介素2受体 内科学
作者
Fangxuan Li,Yao Sun,Jinchao Huang,Wengui Xu,Juntian Liu,Zhi Yong Yuan
出处
期刊:Cancer Medicine [Wiley]
卷期号:8 (17): 7330-7344 被引量:84
标识
DOI:10.1002/cam4.2596
摘要

Abstract Background The tumor microenvironment represents an abnormal niche containing numerous factors, such as T cells, dendritic cells (DCs), regulatory T cells (Tregs), and indoleamine 2,3‐dioxygenase (IDO), involved in maintaining immune homeostasis and tolerance. All these factors may influence the choice of therapy and the clinical outcomes. Methods Flow cytometry was performed to identify CD4+/CD8 + T cells and DCs, and immunohistochemistry was used to evaluate IDO and Forkhead Box P3 (Foxp3) expression; these experiments were performed in order to explore the clinical and prognostic significance of CD4/CD8 + T cells, DCs, Tregs, and IDO expression in gastric carcinoma. Results Smaller tumor size was correlated with higher expression levels of peripheral CD4 + T cells ( P = .003) and CD8 + T cells ( P = .002), and lower IDO expression ( P = .044) in tumors. Well‐differentiated gastric carcinomas displayed higher peripheral ( P = .029) and tumor‐infiltrating CD4 + T cell ( P = .009) populations and a higher tumor‐infiltrating DC1/DC2 ratio ( P = .048). Gastric cancer in the early T stages exhibited higher populations of peripheral DC2s ( P = .044) and a higher tumor‐infiltrating DC1/DC2 ratio ( P = .012). Gastric cancer at the N0 stage had lower tumor‐infiltrating DC2s ( P = .032) and a higher DC1/DC2 ratio ( P = .037). IDO expression was positively correlated with tumor‐infiltrating Foxp3 + Tregs ( P < .001) as well as DC2s ( P < .001), whereas it was negatively correlated with the tumor‐infiltrating CD4/CD8 + T cell ratio ( P = .023). Tumor‐infiltrating Foxp3 + Treg was positively correlated with tumor‐infiltrating DC2s ( r 2 = 0.772; P < .001). At T, N, and TNM stages, the expression levels of peripheral DC2s, tumor‐infiltrating DC1/DC2 ratios, Foxp3 + Tregs, and IDO were significantly correlated with prognosis ( P < .05). The T stage and peripheral DC2s were significant risk factors for overall survival. Conclusion Immunocompetent cells and humoral immune factors, including DC2s, CD4+/CD8 + T cells, Foxp3 + Tregs, and IDO, interact with each other to compose a complex community of tumor immune microenvironment, ultimately affecting tumor progression and survival of gastric cancer.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
秋夜临完成签到,获得积分0
4秒前
龙弟弟完成签到 ,获得积分10
7秒前
阿俊完成签到 ,获得积分10
8秒前
王饱饱完成签到 ,获得积分10
8秒前
9秒前
哈哈哈完成签到,获得积分10
11秒前
Liu丰发布了新的文献求助10
13秒前
SYLH应助Liu丰采纳,获得20
17秒前
CC完成签到,获得积分10
24秒前
科研通AI2S应助Lny采纳,获得10
24秒前
Xx完成签到 ,获得积分10
25秒前
26秒前
loren313完成签到,获得积分0
27秒前
36秒前
myq完成签到 ,获得积分10
46秒前
50秒前
默默灭绝完成签到 ,获得积分10
55秒前
56秒前
怕孤独的香菇完成签到 ,获得积分10
1分钟前
西山菩提完成签到,获得积分10
1分钟前
Ava应助三笠采纳,获得10
1分钟前
啊嚯完成签到,获得积分10
1分钟前
火星上惜天完成签到 ,获得积分10
1分钟前
甜乎贝贝完成签到 ,获得积分10
1分钟前
桐桐应助猪肉水饺采纳,获得10
1分钟前
土豆炖大锅完成签到 ,获得积分10
1分钟前
1分钟前
姆姆没买完成签到 ,获得积分10
1分钟前
zyb完成签到 ,获得积分10
1分钟前
猪肉水饺发布了新的文献求助10
1分钟前
白蓝红完成签到,获得积分10
1分钟前
端庄的萝完成签到,获得积分10
1分钟前
魔幻的妖丽完成签到 ,获得积分10
1分钟前
郑雅柔完成签到 ,获得积分0
1分钟前
1分钟前
科研小石完成签到 ,获得积分10
1分钟前
思源应助科研通管家采纳,获得10
1分钟前
科研通AI2S应助科研通管家采纳,获得10
1分钟前
猪肉水饺完成签到,获得积分10
1分钟前
lisa完成签到 ,获得积分10
1分钟前
高分求助中
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3990836
求助须知:如何正确求助?哪些是违规求助? 3532241
关于积分的说明 11256631
捐赠科研通 3271100
什么是DOI,文献DOI怎么找? 1805313
邀请新用户注册赠送积分活动 882302
科研通“疑难数据库(出版商)”最低求助积分说明 809236