亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整地填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Essential role of the adaptor protein Nck1 in Jurkat T cell activation and function

Jurkat细胞 转染 T细胞 CD28 分子生物学 生物 细胞生物学 小干扰RNA 细胞培养 免疫学 免疫系统 遗传学
作者
Ichaya Yiemwattana,Jatuporn Ngoenkam,Pussadee Paensuwan,R Kriangkrai,Boontharika Chuenjitkuntaworn,Sutatip Pongcharoen
出处
期刊:Clinical and Experimental Immunology [Oxford University Press]
卷期号:167 (1): 99-107 被引量:21
标识
DOI:10.1111/j.1365-2249.2011.04494.x
摘要

The non-catalytic region of tyrosine kinase (Nck) is proposed to play an essential role in T cell activation. However, evidence based on functional and biochemical studies has brought into question the critical function of Nck. Therefore, the aim of the present work was to investigate the role of Nck in T cell activation. To study this, the human Jurkat T cell line was used as a model for human T lymphocytes. The short interfering (si) RNA targeting Nck1 gene was used with electroporation to knock-down Nck1 protein expression in Jurkat T cells. Primary human CD4 T cells were also transfected with the siRNA of Nck1. The results showed that decreased Nck1 protein expression did not affect the apoptosis of the transfected Jurkat T cells compared with control siRNA-transfected cells and non-transfected cells. Upon CD3ε/CD28 stimulation, knock-down of Nck1 in Jurkat T cells caused a decrease in CD69 expression and in interleukin (IL)-2 secretion. Similarly, knock-down of Nck1 in primary CD4 T cells also caused decreased CD69 expression. However, no significant alterations of CD69 and IL-2 expression were found upon phytohaemagglutinin (PHA)/phorbol myristate acetate (PMA) stimulation. Knock-down of Nck1 had no effect on the proliferation of Jurkat T cells stimulated with either PHA or anti-T cell receptor (TCR) monoclonal antibody (C305). The reduced Nck1 expression in Jurkat cells was also associated with a reduced phosphorylation of extracellular regulated kinase (Erk)1 and Erk2 proteins upon CD3ε/CD28 stimulation. In conclusion, the decreased Nck1 protein in Jurkat T cells resulted in an impairment of TCR-CD3-mediated activation involving a defective Erk phosphorylation pathway.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
美好颜发布了新的文献求助10
1秒前
9秒前
大模型应助科研通管家采纳,获得10
13秒前
小二郎应助科研通管家采纳,获得10
13秒前
Betty发布了新的文献求助10
24秒前
33秒前
39秒前
55秒前
1分钟前
慕青应助lty采纳,获得10
1分钟前
1分钟前
1分钟前
lty发布了新的文献求助10
1分钟前
小岩完成签到 ,获得积分10
1分钟前
2分钟前
咕咕发布了新的文献求助10
2分钟前
彩色黑米完成签到 ,获得积分10
2分钟前
科研通AI2S应助科研通管家采纳,获得10
2分钟前
2分钟前
2分钟前
AKi233完成签到,获得积分10
2分钟前
AKi233发布了新的文献求助10
2分钟前
充电宝应助AKi233采纳,获得10
2分钟前
咕咕完成签到,获得积分10
2分钟前
FengyaoWang完成签到,获得积分10
2分钟前
3分钟前
传奇3应助科研通管家采纳,获得10
4分钟前
英俊的铭应助科研通管家采纳,获得10
4分钟前
4分钟前
科研通AI5应助Betty采纳,获得10
4分钟前
MchemG应助彩色的谷云采纳,获得10
4分钟前
吴彦祖完成签到,获得积分10
4分钟前
xixilulixiu完成签到 ,获得积分10
4分钟前
5分钟前
5分钟前
tree发布了新的文献求助10
5分钟前
大模型应助tree采纳,获得10
5分钟前
Lucas应助科研通管家采纳,获得10
6分钟前
科研通AI2S应助科研通管家采纳,获得10
6分钟前
tree完成签到,获得积分20
6分钟前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968504
求助须知:如何正确求助?哪些是违规求助? 3513278
关于积分的说明 11167234
捐赠科研通 3248660
什么是DOI,文献DOI怎么找? 1794386
邀请新用户注册赠送积分活动 875030
科研通“疑难数据库(出版商)”最低求助积分说明 804638