壳聚糖
氟尿嘧啶
MTT法
化学
叶酸受体
体内
叶酸
流式细胞术
纳米颗粒
生物物理学
细胞凋亡
癌细胞
药理学
生物化学
材料科学
分子生物学
纳米技术
化疗
医学
癌症
生物
外科
内科学
生物技术
作者
Dongwei Wang,Yingang Zhang,Menglan Wang,Shasha Luo,Le‐Wei Li,Yibo Yang,Qiufang Zhang,Xiaoping Yang,Chunlian He
标识
DOI:10.1166/jbn.2023.3537
摘要
In this study, we designed a dual-targeting drug formulation that passively targets tumor tissue through the EPR effect and actively targets tumor cells through folic acid binding to folic acid receptor. 5-fluorouracil-loaded folic acid-modified chitosan nanoparticles were prepared by self-assembly, and their average sizes were determined to be 204.7±3.23 nm by dynamic light scattering measurement, and their drug loading and encapsulation rates were calculated to be 15.90% and 47.27%, respectively. MTT assay, flow cytometry and cell migration assays showed that they were more able to inhibit cell viability and cell migration of RKO cells and induce apoptosis than free 5-fluorouracil and 5-fluorouracil-loaded chitosan nanoparticles. The highest uptake efficiency of 5-fluorouracil-loaded folic acid-modified chitosan nanoparticles was observed in RKO cells in the uptake assay. In animal experiments, folic acid-modified nanoparticles inhibited tumor growth more than those of free 5FU and 5-fluorouracil-loaded chitosan nanoparticles, and histological staining results confirmed that they had the highest inhibitory effect on tumor growth. In vivo fluorescence imaging results showed that 5-fluorouracil-loaded chitosan nanoparticles and 5-fluorouracil-loaded folic acid-modified chitosan nanoparticles had good tumor targeting in nude mice, and 5-fluorouracil-loaded folic acid-modified chitosan nanoparticles had stronger targeting.
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