脱氧核酶
化学
滚动圆复制
信号(编程语言)
灵敏度(控制系统)
分析物
对偶(语法数字)
过程(计算)
纳米技术
检出限
计算机科学
色谱法
材料科学
酶
生物化学
电子工程
聚合酶
操作系统
文学类
工程类
艺术
程序设计语言
作者
Ge Xu,Xiaoya Liu,Kuankuan Shao,Xingle Yu,Xiaolin Hu,Xianxian Zhao,Zili Gong
标识
DOI:10.1016/j.aca.2022.340432
摘要
SEVs (small extracellular vesicles) contents signatures appear to mirror pathological changes of diseases, and mapping sEVs contents profile is a promising approach for non-invasive diagnosis of the disease. Herein, we propose a universal system for accurately and damage-freely mapping of sEVs content profile using dual-recognition triggered CHA (catalytic hairpin assembly) and DNAzyme based signal amplification strategy. After immunoassay based capture of CD63 positive sEVs by anti-CD63 lgG coated on the surface of polystyrene plates, probes are incubated with fixed sEVs to penetrate sEVs membrane and act to sense sEVs contents. In detection step, integrated CHA and DNAzyme based strategy is initiated by released initiator from capture probe after recognizing targets, forming a dual circle signal recycling process, realizing signal amplification for high sensitivity. Given the attractive analytical features that i) a universal platform for indistinctive sEVs nucleic acids and protein molecules detection; ii) high sensitivity derived from dual circle signal recycling process; iii) enzyme-free characteristic of integrated CHA and DNAzyme minimizes the interference to sEVs biological activity; iv) mapping of sEVs contents profiles indicates a brand-new strategy for non-invasive diagnosis of the disease, the present approach shows great promise for analyzing additional different analytes in clinical and experimental researches.
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