细胞生物学
间充质
生物
牙本质
成牙本质细胞
Wnt信号通路
连环素
钙粘蛋白
信号转导
上皮
细胞
牙科
生物化学
遗传学
医学
胚胎
作者
Ju‐Kyung Jeong,T.H. Kim,HwaJung Choi,Eui‐Sic Cho
摘要
Abstract Background Wnt/β‐catenin signaling plays a variety of roles in both the dental epithelium and mesenchyme at most stages of tooth development. In this study, we verified the roles of Hertwig's epithelial root sheath (HERS) breakdown in tooth root development. This breakdown results in formation of epithelial cell rests of Malassez (ERM). Results Following induction of β‐catenin stabilization in the epithelium of developing tooth at the moment of HERS breakdown, HERS failed to break down for ERM formation. HERS with stabilized β‐catenin was altered into a multicellular layer enveloping elongated root dentin with higher expression of junctional proteins such as Zo‐1 and E‐cadherin. Importantly, this impairment of HERS breakdown led to arrest of further root elongation. In addition, the portion of root dentin enveloped by the undissociated HERS remained in a hypomineralized state. The odontoblasts showed ectopically higher expression of pyrophosphate regulators including Ank and Npp1, whereas Tnap expression was unchanged. Conclusions Our data suggest that Wnt/β‐catenin signaling is decreased in HERS for ERM formation during root development. Furthermore, ERM formation is important for further elongation and dentin mineralization of the tooth roots. These findings may provide new insight to understand the contribution of ERM to root formation.
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