Analysis of the Chemical Constituents of Rhodobryum roseum Based on UHPLC-Q-Exactive Orbitrap Mass Spectrometry

Objective To further explore the unknown chemical composition and aimed to develop a reliable strategy for characterizing compounds in Rhodobryum roseum ( R. roseum). Methods This study employed an efficient, high-sensitivity, and high-resolution analysis method. The method utilized UHPLC-Q-Exactive Orbitrap MS, along with trace data acquisition mode (parallel reaction monitoring scanning, PRM), and multiple data processing methods. Results A total of 77 chemical substances were identified in R. roseum, including 16 amino acids, 33 organic acids including 6 aromatic acids, 8 phenolics compounds, 2 flavonoid glycosides, 4 flavonoids and 14 other compounds including 4 fatty alcohols by comparing with reference substances, secondary characteristic ion fragments, public databases, and literature. Among these, 74 compounds were found in this plant for the first time. Conclusion This result significantly expands the knowledge of the chemical constituents of R. roseum and contributes to the understanding of its active constituents for quality control.