罗丹明B
检出限
化学
荧光
色谱法
线性范围
罗丹明
选择性
胃蛋白酶
荧光素
酶
有机化学
物理
量子力学
光催化
催化作用
作者
Aya M. Mostafa,Stephen Barton,Stephen P. Wren,James Barker
出处
期刊:Biosensors
[MDPI AG]
日期:2024-03-20
卷期号:14 (3): 151-151
被引量:2
摘要
Two fluorescent molecularly imprinted polymers (MIPs) were developed for pepsin enzyme utilising fluorescein and rhodamine b. The main difference between both dyes is the presence of two (diethylamino) groups in the structure of rhodamine b. Consequently, we wanted to investigate the effect of these functional groups on the selectivity and sensitivity of the resulting MIPs. Therefore, two silica-based MIPs for pepsin enzyme were developed using 3-aminopropyltriethoxysilane as a functional monomer and tetraethyl orthosilicate as a crosslinker to achieve a one-pot synthesis. Results of our study revealed that rhodamine b dyed MIPs (RMIPs) showed stronger binding, indicated by a higher binding capacity value of 256 mg g−1 compared to 217 mg g−1 for fluorescein dyed MIPs (FMIPs). Moreover, RMIPs showed superior sensitivity in the detection and quantitation of pepsin with a linear range from 0.28 to 42.85 µmol L−1 and a limit of detection (LOD) as low as 0.11 µmol L−1. In contrast, FMIPs covered a narrower range from 0.71 to 35.71 µmol L−1, and the LOD value reached 0.34 µmol L−1, which is three times less sensitive than RMIPs. Finally, the developed FMIPs and RMIPs were applied to a separation-free quantification system for pepsin in saliva samples without interference from any cross-reactors.
科研通智能强力驱动
Strongly Powered by AbleSci AI