The simultaneous antibiotics and nitrogen removal in vertical flow constructed wetlands: Effects of substrates and responses of microbial functions

A vertical flow constructed wetland (VFCW) packed with the different substrates was designed to remediate the antibiotics in the wastewater. Zeolite (CW-Z) paralleled with Manganese (Mn) ore (CW-M) and biochar (CW-C) were used to enhance the synchronous removal of ciprofloxacin hydrochloride (CIPH), sulfamethazine (SMZ) and nitrogen (N) from the wastewater. The result indicated that CW-M had a significant potential to remove CIPH (93%), SMZ (69%), TN (71%), NH4+-N (94%) and NO3–-N (94%) across all the treatments. The abundance of amoA, nirK and nirS genes are dramatically higher in CW-M and CW-C, while CW-C inhibited the production of quinolone resistance genes. Results showed that different substrates could affect the microbial diversity and structure. The addition of Mn ore to the water led to an improved abundance of nitrogen-related phyla. Overall, Mn ore has a considerable potential to simultaneously remove antibiotics and N in VFCWs.