清脆的
核酸
基因组编辑
生物
Cas9
DNA
基因组
计算生物学
遗传学
抄写(语言学)
基因
核糖核酸
细胞生物学
分子生物学
语言学
哲学
作者
Haifeng Wang,Morimasa Nakamura,Timothy R. Abbott,David Zhao,Kaiwen Luo,Cordelia Yu,Cindy M. Nguyen,Albert Lo,Timothy Daley,Marie La Russa,Yanxia Liu,Lei S. Qi
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:2019-09-20
卷期号:365 (6459): 1301-1305
被引量:205
标识
DOI:10.1126/science.aax7852
摘要
Tracking nucleic acids in living cells Fluorescence in situ hybridization (FISH) is a powerful molecular technique for detecting nucleic acids in cells. However, it requires cell fixation and denaturation. Wang et al. found that CRISPR-Cas9 protects guide RNAs from degradation in cells only when bound to target DNA. Taking advantage of this target-dependent stability switch, they developed a labeling technique, named CRISPR LiveFISH, to detect DNA and RNA using fluorophore-conjugated guide RNAs with Cas9 and Cas13, respectively. CRISPR LiveFISH improves the signal-to-noise ratio, is compatible with living cells, and allows tracking real-time dynamics of genome editing, chromosome translocation, and transcription. Science , this issue p. 1301
科研通智能强力驱动
Strongly Powered by AbleSci AI