多巴胺能
协议(科学)
神经科学
计算机科学
诱导多能干细胞
生物
多巴胺
医学
胚胎干细胞
生物化学
基因
病理
替代医学
作者
Julie Jerber,James Haldane,Juliette Steer,Daniel Pearce,Minal Patel
标识
DOI:10.17504/protocols.io.bjpgkmjw
摘要
This protocol describes a method for the production of dopaminergic neurons from human iPSCs, using a 52-day long differentiation method adapted from doi.org/10.1038/nature10648. Steps in this protocol assume the use of a single 12 well plate for the purposes of differentiation; adjust volumes accordingly depending on labware used.
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