Detection of Proteins in Serum by Micromagnetic Aptamer PCR (MAP) Technology

Angewandte Chemie International EditionVolume 49, Issue 2 p. 355-358 Communication Detection of Proteins in Serum by Micromagnetic Aptamer PCR (MAP) Technology† Andrew Csordas Dr., Andrew Csordas Dr. Institute for Collaborative Biotechnologies, University of California, Santa Barbara (USA) These authors contributed equally to this work.Search for more papers by this authorAren E. Gerdon Dr., Aren E. Gerdon Dr. Department of Chemistry, Emmanuel College, Boston (USA) These authors contributed equally to this work.Search for more papers by this authorJonathan D. Adams, Jonathan D. Adams Department of Physics, University of California, Santa Barbara (USA)Search for more papers by this authorJiangrong Qian Dr., Jiangrong Qian Dr. Cynvenio Biosystems, Westlake Village (USA)Search for more papers by this authorSeung Soo Oh, Seung Soo Oh Materials Department, Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA)Search for more papers by this authorYi Xiao Dr., Yi Xiao Dr. yixiao@physics.ucsb.edu Materials Department, Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA)Search for more papers by this authorH. Tom Soh Prof., H. Tom Soh Prof. tsoh@engr.ucsb.edu Materials Department, Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA)Search for more papers by this author Andrew Csordas Dr., Andrew Csordas Dr. Institute for Collaborative Biotechnologies, University of California, Santa Barbara (USA) These authors contributed equally to this work.Search for more papers by this authorAren E. Gerdon Dr., Aren E. Gerdon Dr. Department of Chemistry, Emmanuel College, Boston (USA) These authors contributed equally to this work.Search for more papers by this authorJonathan D. Adams, Jonathan D. Adams Department of Physics, University of California, Santa Barbara (USA)Search for more papers by this authorJiangrong Qian Dr., Jiangrong Qian Dr. Cynvenio Biosystems, Westlake Village (USA)Search for more papers by this authorSeung Soo Oh, Seung Soo Oh Materials Department, Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA)Search for more papers by this authorYi Xiao Dr., Yi Xiao Dr. yixiao@physics.ucsb.edu Materials Department, Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA)Search for more papers by this authorH. Tom Soh Prof., H. Tom Soh Prof. tsoh@engr.ucsb.edu Materials Department, Department of Mechanical Engineering, University of California, Santa Barbara, Santa Barbara, CA 93106 (USA)Search for more papers by this author First published: 28 December 2009 https://doi.org/10.1002/anie.200904846Citations: 92 † We are grateful for the financial support from the ARO Institute for Collaborative Biotechnologies, Office of Naval Research, and National Institutes of Health. We thank Xinhui Lou and Jerry Thomas for their invaluable assistance. We also thank Yanting Zhang, Paul Pagano, Marek Turewicz, and André Defusco at Cynvenio Biosystems (Westlake Village) for helpful discussions. Microfabrication was carried out in the Nanofabrication Facility at UCSB. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract On the MAP: The title technique combines microfluidic magnetic sample preparation with highly specific aptamer-based molecular recognition and quantitative PCR. Using platelet-derived growth factor as a model, a fM detection limit was obtained in fetal bovine serum with a dynamic range that spanned five orders of magnitude. Citing Literature Supporting Information Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Filename Description anie_200904846_sm_miscellaneous_information.pdf198.3 KB miscellaneous_information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume49, Issue2January 8, 2010Pages 355-358 RelatedInformation