钙不动杆菌
限制性酶
识别序列
回文序列
生物
回文
等裂体
遗传学
DNA
核酸内切酶
序列(生物学)
分子生物学
不动杆菌
清脆的
细菌
基因
DNA甲基化
基因表达
作者
Abdurashitov Ma,Kiseleva Ev,Miakisheva Tv,Dedkov Vs,Shevchenko Av,Degtiarev CKh
出处
期刊:PubMed
日期:1998-01-24
卷期号:33 (5): 556-8
被引量:1
摘要
The recognition site of a new restriction endonuclease from Acinetobacter calcoaceticus BS was determined. This is a nonpalindromic sequence. AccBSI restrictase cleaves DNA chains in the middle of the recognition sequence; therefore, ligation of its digestion fragments restored AccBSI recognition sites and generated palindromic sequences recognized by SacI and SacII restrictases.
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