基因
外周血单个核细胞
生物
乙型肝炎病毒
免疫系统
先天免疫系统
获得性免疫系统
免疫
基因表达
病毒学
微阵列
病毒
免疫学
遗传学
体外
作者
Yingli He,Yingren Zhao,Shulin Zhang,Yuan Yang,Min Liu,Yan Jin,Jinfeng Liu
摘要
AIM: To investigate peripheral blood mononuclear cells (PBMCs) immune-related gene expression profile in patients with chronic virus hepatitis B(CHB) by oligonucleotide gene array technique. METHODS: PBMCs were collected from the members of a family of clustering hepatitis B virus (HBV) infection including 5 CHB patients and 4 healthy spouses and RNA prepared from PBMCs was hybridized to high-density oligonucleotide arrays(HG-U133A 2.0 Human Gene Chips, Affymetrix), covering the expression of 22 000 human ESTs. Primary scanned image was analyzed with DNT software package. RESULTS: Out of the 22 000 ESTs, 24 different immune-related genes were identified. Among the 24 genes, 7 genes showed increased expression and 17 genes showed decreased expression in CHB compared with those in healthy spouses. The up-regulated genes were mainly associated with adaptive immunity, while the down-regulated genes were associated with innate immunity. CONCLUSION: Our findings suggest that HBV infection alters a broad range of immunity genes expression and innate immunity-associated genes are important in the defense against HBV chronic infection.
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