蛋白质组
病理
免疫印迹
蛋白质组学
唾液腺
生物
腮腺
膜联蛋白A2
分子生物学
骨膜炎
多形性腺瘤
内科学
膜联蛋白
医学
生物信息学
生物化学
染色
基因
细胞外基质
作者
Veronica Seccia,Elena Navari,Elena Donadio,Claudia Boldrini,Federica Ciregia,Maurizio Ronci,Antonio Aceto,Iacopo Dallan,Antonio Lucacchini,Augusto Pietro Casani,Maria Rosa Mazzoni,Laura Giusti
标识
DOI:10.1007/s12105-019-01040-2
摘要
The purpose of this study was to define the proteome profile of fine needle aspiration (FNA) samples of malignant major salivary gland tumors (MSGT) compared to benign counterparts, and to evaluate potential clinical correlations and future applications. Patients affected by MSGT (n = 20), pleomorphic adenoma (PA) (n = 37) and Warthin’s tumor (WT) (n = 14) were enrolled. Demographic, clinical and histopathological data were registered for all patients. FNA samples were processed to obtain the protein extracts. Protein separation was obtained by two-dimensional electrophoresis (2-DE) and proteins were identified by mass spectrometry. Western blot analysis was performed to validate the 2-DE results. Statistical differences between groups were calculated by the Mann–Whitney U test for non-normal data. Spearman’s rank correlation coefficient was calculated to evaluate correlations among suggested protein biomarkers and clinical parameters. Twelve and 27 differentially expressed spots were found for MSGT versus PA and MSGT versus WT, respectively. Among these, annexin-5, cofilin-1, peptidyl-prolyl-cis–trans-isomerase-A and F-actin-capping-alpha-1 were able to differentiate MSGT from PA, WT, and healthy samples. Moreover, STRING analysis suggested cofilin-1 as a key node of protein interactions. Some of the overexpressed proteins are related to some clinical factors of our cohort, such as survival and outcome. Our results suggest potential protein biomarkers of MSGT, which could allow for more appropriate treatment plans, as well as shedding light on the molecular pathways involved.
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