Ratiometric fluorescence sensor based on cholesterol oxidase-functionalized mesoporous silica nanoparticle@ZIF-8 core-shell nanocomposites for detection of cholesterol

胆固醇氧化酶 化学 荧光 检出限 介孔二氧化硅 纳米颗粒 生物传感器 量子点 纳米复合材料 介孔材料 吸附 纳米传感器 猝灭(荧光) 分子 核化学 纳米技术 色谱法 有机化学 胆固醇 材料科学 生物化学 催化作用 物理 量子力学
作者
Ke Wang,Hailong Ren,Nan Li,Xiaoyan Tan,Fuquan Dang
出处
期刊:Talanta [Elsevier BV]
卷期号:188: 708-713 被引量:60
标识
DOI:10.1016/j.talanta.2018.06.019
摘要

A novel ratiometric fluorescence sensing system based on cholesterol oxidase-functionalized dual-color mesoporous silica nanoparticles (MSNs)@metal-organic framework core-shell nanocomposite is demonstrated for cholesterol detection. MSNs were first loaded with 5-aminofluorescein (AF) inside pores and then wrapped with red-emission CdTe quantum dots (QDs) on the surface to seal in the dye molecules, forming the signal displaying unit (AF-MSN-QDs). Next, AF-MSN-QDs were encapsulated with zeolitic imidazolate framework (ZIF-8) to form a transition layer with distinct size-selectivity, which not only protected the cores from corrosion but also greatly decreased background interference from large molecules. More significantly, the ZIF-8 shells showed high affinity for most enzymes, which made it possible for cholesterol oxidase (ChOx) to self-organize on the surface of ZIF-8-encapsulated AF-MSN-QDs via chemo-physical adsorption, forming novel core-shell nanocomposites ([email protected]) as a sensing platform for cholesterol detection. The detectable signal was monitored by enzymatic product-quenching fluorescence of the QDs. The fluorescence changes of I520/I618 showed excellent linearity with H2O2 concentrations in the range of 5–100 nM, with a limit of detection (LOD) as low as 0.89 nM. As a proof-of-concept, cholesterol was selectively detected with beneficial LOD as low as 0.923 μg/mL, demonstrating the great potential of this biosensor platform for other biologically important molecules with H2O2-producing oxidases.
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