生物
多巴胺能
啮齿动物
中脑
分离(微生物学)
细胞生物学
神经科学
细胞培养
啮齿动物模型
多巴胺
中枢神经系统
内分泌学
生态学
遗传学
微生物学
作者
Jan Pruszak,Lothar Just,Ole Isacson,Guido Nikkhah
标识
DOI:10.1002/9780470151808.sc02d05s11
摘要
Abstract The ability to isolate ventral midbrain (VM) precursor cells and neurons provides a powerful means to characterize their differentiation properties and to study their potential for restoring dopamine (DA) neurons degenerated in Parkinson's disease (PD). Preparation and maintenance of DA VM in primary culture involves a number of critical steps to yield healthy cells and appropriate data. Here, we offer a detailed description of protocols to consistently prepare VM DA cultures from rat and mouse embryonic fetal‐stage midbrain. We also present methods for organotypic culture of midbrain tissue, for differentiation as aggregate cultures, and for adherent culture systems of DA differentiation and maturation, followed by a synopsis of relevant analytical read‐out options. Isolation and culture of rodent VM precursor cells and DA neurons can be exploited for studies of DA lineage development, of neuroprotection, and of cell therapeutic approaches in animal models of PD. Curr. Protoc. Stem Cell Biol . 11:2D.5.1‐2D.5.21. © 2009 by John Wiley & Sons, Inc.
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