化学
儿茶酚
酪氨酸酶
儿茶酚氧化酶
苯醌
对苯二酚
酶
核化学
有机化学
光化学
多酚氧化酶
过氧化物酶
作者
Shunichi Uchiyama,Yasushi Hasebe,Hiroya Shimizu,Hideki Ishihara
标识
DOI:10.1016/0003-2670(93)80402-7
摘要
A cyclic reaction between catechol and 1,2-benzoquinone takes place by combining the tyrosinase reaction and the chemical reduction of 1,2-benzoquinone to catechol by l-ascorbic acid. Catechol is oxidized by the dissolved oxygen catalysed by the enzyme and its consumption is not compensated for by the chemical regeneration of catechol from 1,2-benzoquinone. The dissolved oxygen thus continues to decrease during the cyclic reaction and consequently the decrease in the reduction current of oxygen is amplified. The amplification factor of 1 X 10−6 M catechol at 0.01 M l-ascorbate and pH 7.0 was found to be 300 when enzyme activity was 20 U ml−1 and the reaction time was 10 min. The calibration graph for catechol with a potato tissue membrane electrode was moved in parallel to a lower concentration range and the detection limit was found to be 5 X 10−8 M (amplification factor 400) when buffer solution of pH 7.0 containing 0.01 M l-ascorbic acid was used.
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