Simultaneous quantitation of arecoline, acetylcholine, and choline in tissue using gas chromatography/electron impact mass spectrometry

Biological Mass SpectrometryVolume 21, Issue 6 p. 299-304 Article Simultaneous quantitation of arecoline, acetylcholine, and choline in tissue using gas chromatography/electron impact mass spectrometry T. A. Patterson, T. A. Patterson College of Pharmacy, University of South Carolina, Columbia, South Carolina 29208, USASearch for more papers by this authorJ. W. Kosh, Corresponding Author J. W. Kosh College of Pharmacy, University of South Carolina, Columbia, South Carolina 29208, USACollege of Pharmacy, University of South Carolina, Columbia, South Carolina 29208, USASearch for more papers by this author T. A. Patterson, T. A. Patterson College of Pharmacy, University of South Carolina, Columbia, South Carolina 29208, USASearch for more papers by this authorJ. W. Kosh, Corresponding Author J. W. Kosh College of Pharmacy, University of South Carolina, Columbia, South Carolina 29208, USACollege of Pharmacy, University of South Carolina, Columbia, South Carolina 29208, USASearch for more papers by this author First published: June 1992 https://doi.org/10.1002/bms.1200210606Citations: 27AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract A capillary gas chromatography/mass spectrometry (GC/MS) assay for the simultaneous quantitation of arecoline (ARE), acetylcholine (ACh), and choline (Ch) in biological tissue has been developed. The method utilizes hexa-deuterated ARE and nonadeuterated ACh and Ch as internal standards. The compounds were ion-pair extracted from tissue using sodium tetraphenylboron in 3-heptanone. GC/MS analysis was achieved using capillary GC and electron impact mass spectrometry. Quantitation was accomplished using selected ion monitoring at m/z 140 and 146 for non-deuterated and deuterated arecoline respectively, and m/z 58 and 64 for non-deuterated and deuterated ACh and Ch respectively. The method easily detected 25 pmol of all three compounds taken through the assay, and was linear through 50 nmol. References 1 W. Marmé, Ther. Monatsh. 4, 291 (1890). Google Scholar 2 U. S. von Euler and B. 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