动力蛋白
中国仓鼠卵巢细胞
化学
耳蜗
聚合物囊泡
转染
生物物理学
细胞生物学
绿色荧光蛋白
毛细胞
分子生物学
生物化学
生物
解剖
受体
基因
有机化学
两亲性
聚合物
共聚物
作者
Elena V. Surovtseva,A. Johnston,Weikai Zhang,Ya Zhang,Adele Kim,Michio Murakoshi,Hiroshi Wada,Tracey A. Newman,Jing Zou,Ilmari Pyykkö
标识
DOI:10.1016/j.ijpharm.2011.12.042
摘要
Targeted delivery of treatment agents to the inner ear using nanoparticles is an advanced therapeutic approach to cure or alleviate hearing loss. Designed to target the outer hair cells of the cochlea, two 12-mer peptides (A665 and A666) with affinity to prestin were identified following 3 rounds of sequential phage display. Two-round display with immobilized prestin protein was used to enrich the library for full-length prestin. The last round was performed using Cos-7 cells transiently transfected with a cCFP-prestin plasmid to display phages expressing peptides restrictive to the extracellular loops of prestin. The binding properties of A665 and A666 shown by flow cytometry demonstrated selectivity to prestin-expressing Chinese hamster ovary cells. PEG6K-b-PCL19K polymersomes covalently labelled with these peptides demonstrated effective targeting to outer hair cells in a rat cochlear explant study.
科研通智能强力驱动
Strongly Powered by AbleSci AI