Deletion of the Gly600 Residue of Alicyclobacillus acidocaldarius Squalene Cyclase Alters the Substrate Specificity into that of the Eukaryotic‐Type Cyclase Specific to (3S)‐2,3‐Oxidosqualene

Removal service: A deletion mutant lacking the Gly600 residue of a prokaryotic squalene–hopene cyclase was prepared. Surprisingly, the mutant cyclase has no enzyme activity for 1 and 3, but shows a high conversion ratio for 2 (see scheme). Deleting Gly600 alters the specificity from prokaryotic into that of eukaryotic-type cyclases. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2004/z461523_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.