小胶质细胞
化学
多巴胺能
神经科学
多巴胺
胞吐
炎症
细胞生物学
生物物理学
纳米技术
生物化学
分泌物
生物
免疫学
材料科学
作者
Fuli Zhang,Yun Tang,Hongwei� Jiang,Xiaoke Yang,Wei‐Hua Huang
标识
DOI:10.1002/cjoc.202100178
摘要
Main observation and conclusion Parkinson's disease (PD) is one of the most common neurogenerative diseases (NDDs), characterized as less neurotransmitter release and loss of dopaminergic (DAergic) neurons with microglial inflammatory response as a key player. Natural product harpagide with anti‐inflammatory function is a potential therapeutic drug of PD, but its role towards microglial activation and inflammation‐mediated neuronal injury remained unsure. In this work, taking advantage of nanoelectrode amperometry with high temporal‐spatial resolution, we used nanowire electrodes (NWEs) to monitor intracellular reactive oxygen species (ROS) level and carbon fiber nanoelectrodes (CFNEs) to detect synaptic dopamine exocytosis, to explore the effect of harpagide in modulating microglial inflammatory reaction and protecting DAergic neurons in neuron‐microglia co‐culture system. The results indicate that harpagide inhibits microglia from activation induced by LPS/IFN‐γ and generation of ROS, therefore reduces inflammation‐mediated neural injury and maintains dopamine exocytosis function. These conclusions establish that harpagide possesses promising avenues for preventive or therapeutic interventions against PD and other NDDs.
科研通智能强力驱动
Strongly Powered by AbleSci AI