材料科学
自愈水凝胶
明胶
流变学
脚手架
组织工程
聚合物
化学工程
复合材料
生物医学工程
高分子化学
化学
生物化学
医学
工程类
作者
Thomas Kreller,Thomas Distler,Susanne Heid,Stefan Gerth,Rainer Detsch,Aldo R. Boccaccını
标识
DOI:10.1016/j.matdes.2021.109877
摘要
This work explored 3D printing to mimic the intrinsic hierarchical structure of natural articular cartilage. Alginate di-aldehyde- gelatine (ADA-GEL) hydrogel was used as ink to create hierarchically ordered scaffolds. In comparison to previously reported ADA-GEL compositions, we introduce a modified formulation featuring increased amounts of thermally modified gelatine. Gelatine was degraded by hydrolysis which resulted in tailorable printability characteristics further substantiated by rheological analysis. ADA (3.75 %w/v)-GEL (7.5 %w/v) with gelatine modified at 80 °C for 3 h could be printed in hierarchical complex structures reaching scaffold heights of over 1 cm. The hierarchical structure of the scaffolds was confirmed via µCT analysis. To examine mechanical properties as well as the suitability of the hydrogel as a proper matrix for cell seeding and encapsulation, nanoindentation was performed. Elastic moduli in the range of ~ 5 kPa were measured. Gelatine heat pre-treatment resulted in modifiable mechanical and rheological characteristics of ADA-GEL. In summary, this study demonstrates the possibility to enhance the printability of ADA-GEL hydrogels to fabricate hierarchical scaffold structures with shape stability and fidelity, without the necessity to change the initial hydrogel chemistry by the use of additives or crosslinkers, providing a valuable approach for fabrication of designed scaffolds for cartilage tissue engineering.
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