糖酵解
己糖激酶
癌症研究
细胞凋亡
肝癌
生物
细胞培养
癌细胞
细胞生长
肝细胞癌
体内
生物化学
癌症
酶
化学
细胞生物学
分子生物学
遗传学
作者
Dan Liú,Xiaowen Zhou,Sen Wang,Tingting Dai,Lixia Luo,Qing Ning,Bojia Liu,Ziyu Jiang,Nan Yao,Yi Luo,Jie Song
标识
DOI:10.1016/j.jff.2021.104782
摘要
Balanophora laxiflora Hemsl has been traditionally used to treat liver cancer. Eight ingredients were identified in the ethanol extracts of Balanophora laxiflora Hemsl (BLE) by UHPLC-Q-TOF-MS/MS analysis. BLE inhibited viability and migration of HepG2 and Hepa1-6 cells, but triggered negligible apoptosis. BLE also impeded glycolysis, glucose uptake and lactate generation. Hexokinase II (HKII), a key enzyme involved in glycolysis, was significantly repressed by BLE. HKII plasmid in HepG2 enhanced cell migration, glucose uptake and cell proliferation, which were all abrogated by BLE pre-treatment. BLE incubation also down-regulated the migration and glycolysis in HKII-overexpressed Hepa1-6 cells. The anti-cancer effect of BLE in vivo was evaluated. BLE inhibited the metastasis of H22 cells in lung and alleviated orthotopic liver cancer induced by diethylnitrosamine in rats. BLE also repressed tumor growth in mice bearing HepG2 cell xenografts. Therefore, BLE can be considered as a new drug candidate for the treatment of liver cancer.
科研通智能强力驱动
Strongly Powered by AbleSci AI