LC determination of cephalosporins in in vitro rat intestinal sac absorption model

化学 色谱法 高效液相色谱法 碳酸氢盐 剂型 反相色谱法 吸收(声学) 磁导率 分析化学(期刊) 生物化学 有机化学 物理 声学
作者
Pradeep Sharma,H. P. S. CHAWLA,Ramesh Panchagnula
出处
期刊:Journal of Pharmaceutical and Biomedical Analysis [Elsevier BV]
卷期号:27 (1-2): 39-50 被引量:41
标识
DOI:10.1016/s0731-7085(01)00506-4
摘要

Cefotaxime sodium (CX) and Ceftazidime pentahydrate (CZ) are peptidomimetic cephalosporins (CPS) which exist as zwitterionic compounds at physiological pH and because of this reason they are not absorbed appreciably on peroral administration. The permeability of these compounds can be increased transiently by altering membrane characteristics of absorptive epithelium by use of sorption promoters (SPs). In present work a simple validated HPLC method utilizing isocratic mobile phase and having short retention times for CX and CZ is developed which can be used to monitor their concentrations in Kreb's Ringer Bicarbonate (KRB) solution in in vitro intestinal sac absorption model. The same was utilized to determine apparent permeability coefficients and absorption profiles of CPS by modified Wilson–Wiseman method. The CPS were analysed by the reverse phase HPLC method using Shim-pack C18 column. The mobile phase used was of isocratic composition with phosphate buffer (pH 7.0, 3.5 g/l of KH2PO4 dissolved in 0.03 M Na2HPO4 · 2H2O) and methanol in proportion 85:15 for CZ and 70:30 for CX. The flow rate was 1ml/min and quantitative determinations were carried out at 254 nm at 25 °C. The method was found specific because none of the proposed SPs, components of KRB and intestinal sac artefacts interfered with the drug peaks. The drug concentration versus area under peak relationship was found to be linear in concentration range of 0.25–20.0 μg/ml. The recovery studies, intraday variation, interday variation and interanalyst variation were within statistical limits. The limit of detection (LOD) was 95.0 and 100.0 ng/ml for CZ and CX, respectively. The limit of Quantitation (LOQ) was 240.0 and 250.0 ng/ml for CZ and CX, respectively. The proposed method was found to be rapid and selective and hence applied for continuous monitoring of CPS in in vitro intestinal sac absorption studies.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
顺利萃发布了新的文献求助30
刚刚
皮老师发布了新的文献求助10
1秒前
1秒前
fanmo完成签到 ,获得积分0
2秒前
2秒前
聪慧的小土豆完成签到 ,获得积分10
4秒前
4秒前
自觉的凌青完成签到,获得积分10
4秒前
6秒前
8秒前
畅快芝麻发布了新的文献求助10
8秒前
zriverm发布了新的文献求助10
10秒前
干煸鸡发布了新的文献求助10
11秒前
12秒前
13秒前
13秒前
14秒前
量子星尘发布了新的文献求助10
14秒前
呸呸晓鹏发布了新的文献求助10
18秒前
枫之林发布了新的文献求助10
19秒前
小蘑菇应助zriverm采纳,获得10
21秒前
21秒前
21秒前
SciGPT应助小鱼采纳,获得10
22秒前
学术渣渣发布了新的文献求助30
22秒前
渡劫完成签到,获得积分10
23秒前
23秒前
26秒前
靓丽雨梅完成签到 ,获得积分10
26秒前
等待的花生完成签到,获得积分10
26秒前
28秒前
Mangues发布了新的文献求助30
28秒前
呸呸晓鹏完成签到,获得积分20
28秒前
搜集达人应助xuxu采纳,获得10
29秒前
111111关注了科研通微信公众号
30秒前
30秒前
30秒前
小唐尼发布了新的文献求助30
34秒前
34秒前
38秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
ACSM’s Guidelines for Exercise Testing and Prescription, 12th edition 500
‘Unruly’ Children: Historical Fieldnotes and Learning Morality in a Taiwan Village (New Departures in Anthropology) 400
Indomethacinのヒトにおける経皮吸収 400
Phylogenetic study of the order Polydesmida (Myriapoda: Diplopoda) 370
基于可调谐半导体激光吸收光谱技术泄漏气体检测系统的研究 350
Robot-supported joining of reinforcement textiles with one-sided sewing heads 320
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3989069
求助须知:如何正确求助?哪些是违规求助? 3531351
关于积分的说明 11253589
捐赠科研通 3269939
什么是DOI,文献DOI怎么找? 1804851
邀请新用户注册赠送积分活动 882074
科研通“疑难数据库(出版商)”最低求助积分说明 809073