亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Culture supernatant produced by Lactobacillus kefiri from kefir inhibits the growth of Cronobacter sakazakii

阪崎克罗诺杆菌 益生菌 食品科学 抗菌剂 开菲尔 微生物学 发酵 乳酸 细菌 微生物 化学 生物 婴儿配方奶粉 遗传学
作者
Dong‐Hyeon Kim,Dana Jeong,Kwang‐Young Song,Inpil Kang,Hyun‐Sook Kim,Kun‐Ho Seo
出处
期刊:Journal of Dairy Research [Cambridge University Press]
卷期号:85 (1): 98-103 被引量:23
标识
DOI:10.1017/s0022029917000802
摘要

Cronobacter sakazakii is a life-threatening foodborne pathogen found in powdered infant formula and dairy products. Kefir is a dairy probiotic product and its antimicrobial activity against C. sakazakii was reported in our previous study. To identify key microorganisms that mediate growth suppression, we tested the antimicrobial activity of culture supernatants derived from lactic acid bacteria found in kefir. Lactobacillus kefiri DH5, L. kefiranofaciens DH101, and B ifidobacterium longum 7 20 (a commercial probiotic strain that served as a positive control) all significantly inhibited the growth of C. sakazakii ATCC 29544, delaying the initiation of exponential growth from 3 to 9 h in the nutrient broth. Among them, L. kefiri DH5 exerted the strongest antimicrobial effects against C. sakazakii , showing bactericidal effect at the addition of 300 µl of supernatant in 1 ml of nutrient broth. Interestingly, the supernatant of L. kefiri DH5 has higher pH and lower titrable acidity than that of L. kefiranofaciens DH101, suggesting metabolites produced by heterofermentation of L. kefiri acted more effectively to antagonise the growth of C. sakazakii . In addition, the supernatant of L. kefiri DH5 induced the leakage of cytoplasmic materials including nucleic acid and proteins, suggesting L. kefiri DH5 disrupted the cellular membrane integrity of C. sakazakii . Considering that pH neutralisation reduced the L. kefiri -dependent growth suppression, it is inferred that this activity is mainly due to organic acids produced during the fermentation process.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
bkagyin应助莘莘采纳,获得10
4秒前
18秒前
pjh发布了新的文献求助10
19秒前
35秒前
Raunio完成签到,获得积分10
38秒前
44秒前
48秒前
zqq完成签到,获得积分0
51秒前
1分钟前
19900420发布了新的文献求助10
1分钟前
1分钟前
1分钟前
1分钟前
西瓜撞地球完成签到,获得积分20
1分钟前
1分钟前
充电宝应助19900420采纳,获得10
1分钟前
NexusExplorer应助llm采纳,获得10
1分钟前
1分钟前
1分钟前
1分钟前
pjh发布了新的文献求助30
2分钟前
快乐小菜瓜完成签到 ,获得积分10
2分钟前
2分钟前
身法马可波罗完成签到 ,获得积分10
2分钟前
bkagyin应助pjh采纳,获得10
2分钟前
llm发布了新的文献求助10
2分钟前
pjh完成签到,获得积分10
2分钟前
2分钟前
zs完成签到 ,获得积分10
2分钟前
2分钟前
2分钟前
科研小黑发布了新的文献求助10
3分钟前
dolphin完成签到 ,获得积分0
3分钟前
彭于晏应助xxxc采纳,获得10
3分钟前
3分钟前
3分钟前
Jasmine发布了新的文献求助10
3分钟前
3分钟前
3分钟前
阿智发布了新的文献求助10
3分钟前
高分求助中
歯科矯正学 第7版(或第5版) 1004
Semiconductor Process Reliability in Practice 1000
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 600
GROUP-THEORY AND POLARIZATION ALGEBRA 500
Mesopotamian divination texts : conversing with the gods : sources from the first millennium BCE 500
Days of Transition. The Parsi Death Rituals(2011) 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3234546
求助须知:如何正确求助?哪些是违规求助? 2880894
关于积分的说明 8217297
捐赠科研通 2548495
什么是DOI,文献DOI怎么找? 1377792
科研通“疑难数据库(出版商)”最低求助积分说明 647999
邀请新用户注册赠送积分活动 623347