The spatial relationship between stem cells and their progeny in the basal layer of human epidermis: a new view based on whole-mount labelling and lineage analysis

生物 表皮(动物学) 干细胞 细胞生物学 整合素 人口 克隆(Java方法) 角质形成细胞 干细胞标记物 基础(医学) 分子生物学 细胞 细胞培养 解剖 遗传学 基因 人口学 社会学 内分泌学 胰岛素
作者
Uffe Birk Jensen,Sally Lowell,Fiona M. Watt
出处
期刊:Development [The Company of Biologists]
卷期号:126 (11): 2409-2418 被引量:359
标识
DOI:10.1242/dev.126.11.2409
摘要

ABSTRACT In order to examine the spatial organisation of stem cells and their progeny in human epidermis, we developed a method for whole-mount epidermal immunofluorescence labelling using high surface β1 integrin expression as a stem cell marker. We confirmed that there are clusters of high β1 integrin-expressing cells at the tips of the dermal papillae in epidermis from several body sites, whereas α6 integrin expression is more uniform. The majority of actively cycling cells detected by Ki67 or bromodeoxyuridine labelling were found in the β1 integrindull, transit amplifying population and integrin-negative, keratin 10-positive cells left the basal layer exclusively from this compartment. When we examined p53-positive clones in sun-exposed epidermis, we found two types of clone that differed in size and position in a way that was consistent with the founder cell being a stem or transit amplifying cell. The patterning of the basal layer implies that transit amplifying cells migrate over the basement membrane away from the stem cell clusters. In support of this, isolated β1 integrin-dull keratinocytes were more motile on type IV collagen than β1 integrin-bright keratinocytes and EGFP-labelled stem cell clones in confluent cultured sheets were compact, whereas transit amplifying clones were dispersed. The combination of whole-mount labelling and lineage marking thus reveals features of epidermal organisation that were previously unrecognised.

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