已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Development of low-cost cage-like particles to formulate veterinary vaccines

卵清蛋白 蛋黄 明矾 佐剂 薄层色谱法 色谱法 抗体 免疫系统 生物 化学 食品科学 免疫学 有机化学
作者
Giuliana Lupi,Florencia X. Santiago Valtierra,Gabriel Cabrera,Roque Spinelli,Álvaro S. Siano,Verónica González,Antonio Osuna,Gerardo M. Oresti,Iván Marcipar
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier]
卷期号:251: 110460-110460
标识
DOI:10.1016/j.vetimm.2022.110460
摘要

Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extraction and by dialyzing a non-refined saponins extract, respectively. Lipids were fully characterized by thin layer chromatography (TLC) and gas-chromatography (GC) and enzymatic methods, and saponins were characterized by TLC, HPLC and MALDI-TOF. Cage-like particles were prepared with these components or with commercial inputs. Both particles and the traditional Alum used in veterinary vaccines were compared by immunizing mice with Ovalbumin (OVA) formulated with these adjuvants and assessing IgG1, IgG2a anti OVA antibodies and specific Delayed-type Hypersensitivity (DTH). In the yolk extract, a mixture of phospholipids, cholesterol and minor components of the extract (e.g. lyso-phospholipids) with suitable proportions to generate cage-like particles was obtained. Also, semi-purified saponins with similar features to those of the QuilA® were obtained. Cage-like particles prepared with these components have 40-50 nm and triggers similar levels of Anti-OVA IgG1 and DTH than with commercial inputs but higher specific-IgG2a. Both adjuvants largely increased the levels of IgG1, IgG2a and DTH in relation to the formulation with Alum. The methods described to extract lipids from egg yolk and saponins from non-refined extract allowed us to obtain an inexpensive and highly effective adjuvant.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
哈哈完成签到 ,获得积分10
3秒前
3秒前
w5566完成签到 ,获得积分10
3秒前
4秒前
深情安青应助蜉蝣采纳,获得10
4秒前
5秒前
共产主义接班人完成签到,获得积分10
6秒前
mangle完成签到,获得积分10
9秒前
Bearbiscuit发布了新的文献求助10
10秒前
充电宝应助韩月采纳,获得10
11秒前
甜美帅哥完成签到,获得积分10
11秒前
wang5945完成签到 ,获得积分10
11秒前
Atropine完成签到 ,获得积分10
12秒前
Jasper应助宝宝熊的熊宝宝采纳,获得10
13秒前
寻道图强应助科研通管家采纳,获得200
14秒前
maction应助科研通管家采纳,获得10
14秒前
从容芮应助科研通管家采纳,获得20
14秒前
领导范儿应助科研通管家采纳,获得10
14秒前
NexusExplorer应助科研通管家采纳,获得10
14秒前
寻道图强应助科研通管家采纳,获得30
14秒前
JamesPei应助科研通管家采纳,获得10
14秒前
14秒前
慕青应助科研通管家采纳,获得30
14秒前
顾矜应助科研通管家采纳,获得10
14秒前
19秒前
19秒前
23秒前
朴素若枫发布了新的文献求助10
28秒前
小马甲应助hujing采纳,获得10
29秒前
hhhhh完成签到 ,获得积分10
32秒前
33秒前
keyanzhang发布了新的文献求助10
34秒前
35秒前
sss完成签到 ,获得积分10
38秒前
Yz完成签到 ,获得积分10
39秒前
39秒前
朴素若枫完成签到,获得积分10
40秒前
hujing发布了新的文献求助10
41秒前
Yam完成签到,获得积分10
42秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
Dictionary of socialism 350
Mixed-anion Compounds 300
Geochemistry, 2nd Edition 地球化学经典教科书第二版 300
Idoxuridine 260
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3196567
求助须知:如何正确求助?哪些是违规求助? 2845266
关于积分的说明 8053827
捐赠科研通 2509906
什么是DOI,文献DOI怎么找? 1342121
科研通“疑难数据库(出版商)”最低求助积分说明 639304
邀请新用户注册赠送积分活动 608608