Development of low-cost cage-like particles to formulate veterinary vaccines

卵清蛋白 蛋黄 明矾 佐剂 薄层色谱法 色谱法 抗体 免疫系统 生物 化学 食品科学 免疫学 有机化学
作者
Giuliana Lupi,Florencia X. Santiago Valtierra,Gabriel Cabrera,Roque Spinelli,Álvaro S. Siano,Verónica González,Antonio Osuna,Gerardo M. Oresti,Iván Marcipar
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier]
卷期号:251: 110460-110460
标识
DOI:10.1016/j.vetimm.2022.110460
摘要

Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extraction and by dialyzing a non-refined saponins extract, respectively. Lipids were fully characterized by thin layer chromatography (TLC) and gas-chromatography (GC) and enzymatic methods, and saponins were characterized by TLC, HPLC and MALDI-TOF. Cage-like particles were prepared with these components or with commercial inputs. Both particles and the traditional Alum used in veterinary vaccines were compared by immunizing mice with Ovalbumin (OVA) formulated with these adjuvants and assessing IgG1, IgG2a anti OVA antibodies and specific Delayed-type Hypersensitivity (DTH). In the yolk extract, a mixture of phospholipids, cholesterol and minor components of the extract (e.g. lyso-phospholipids) with suitable proportions to generate cage-like particles was obtained. Also, semi-purified saponins with similar features to those of the QuilA® were obtained. Cage-like particles prepared with these components have 40-50 nm and triggers similar levels of Anti-OVA IgG1 and DTH than with commercial inputs but higher specific-IgG2a. Both adjuvants largely increased the levels of IgG1, IgG2a and DTH in relation to the formulation with Alum. The methods described to extract lipids from egg yolk and saponins from non-refined extract allowed us to obtain an inexpensive and highly effective adjuvant.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
研友_VZG7GZ应助科研通管家采纳,获得10
1秒前
无为应助科研通管家采纳,获得10
1秒前
科研通AI2S应助科研通管家采纳,获得10
1秒前
1秒前
Orange应助科研通管家采纳,获得10
1秒前
科研通AI2S应助科研通管家采纳,获得10
1秒前
思源应助科研通管家采纳,获得10
2秒前
FATHER LI完成签到,获得积分10
2秒前
共享精神应助科研通管家采纳,获得10
2秒前
无为应助科研通管家采纳,获得10
2秒前
所所应助科研通管家采纳,获得10
2秒前
小二郎应助科研通管家采纳,获得10
2秒前
丘比特应助科研通管家采纳,获得10
2秒前
2秒前
打打应助科研通管家采纳,获得10
2秒前
大个应助科研通管家采纳,获得10
2秒前
无私的盼望完成签到 ,获得积分10
2秒前
灵巧墨镜发布了新的文献求助30
2秒前
Sink完成签到,获得积分10
3秒前
4秒前
5秒前
5秒前
5秒前
星辰大海应助大方百招采纳,获得10
7秒前
李斌发布了新的文献求助10
8秒前
9秒前
小鱼发布了新的文献求助10
9秒前
艳艳子发布了新的文献求助10
10秒前
10秒前
11秒前
陈12发布了新的文献求助10
12秒前
12秒前
某某完成签到,获得积分10
13秒前
14秒前
15秒前
李半斤发布了新的文献求助10
15秒前
NexusExplorer应助艳艳子采纳,获得10
16秒前
去给劳资读文献应助evacqy采纳,获得10
17秒前
文献通完成签到,获得积分10
17秒前
洛洛完成签到,获得积分10
17秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3183068
求助须知:如何正确求助?哪些是违规求助? 2833280
关于积分的说明 7993179
捐赠科研通 2495413
什么是DOI,文献DOI怎么找? 1331490
科研通“疑难数据库(出版商)”最低求助积分说明 636300
邀请新用户注册赠送积分活动 603474