Development of low-cost cage-like particles to formulate veterinary vaccines

卵清蛋白 蛋黄 明矾 佐剂 薄层色谱法 色谱法 抗体 免疫系统 生物 化学 食品科学 免疫学 有机化学
作者
Giuliana Lupi,Florencia X. Santiago Valtierra,Gabriel Cabrera,Roque Spinelli,Álvaro S. Siano,Verónica González,Antonio Osuna,Gerardo M. Oresti,Iván Marcipar
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier]
卷期号:251: 110460-110460
标识
DOI:10.1016/j.vetimm.2022.110460
摘要

Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extraction and by dialyzing a non-refined saponins extract, respectively. Lipids were fully characterized by thin layer chromatography (TLC) and gas-chromatography (GC) and enzymatic methods, and saponins were characterized by TLC, HPLC and MALDI-TOF. Cage-like particles were prepared with these components or with commercial inputs. Both particles and the traditional Alum used in veterinary vaccines were compared by immunizing mice with Ovalbumin (OVA) formulated with these adjuvants and assessing IgG1, IgG2a anti OVA antibodies and specific Delayed-type Hypersensitivity (DTH). In the yolk extract, a mixture of phospholipids, cholesterol and minor components of the extract (e.g. lyso-phospholipids) with suitable proportions to generate cage-like particles was obtained. Also, semi-purified saponins with similar features to those of the QuilA® were obtained. Cage-like particles prepared with these components have 40-50 nm and triggers similar levels of Anti-OVA IgG1 and DTH than with commercial inputs but higher specific-IgG2a. Both adjuvants largely increased the levels of IgG1, IgG2a and DTH in relation to the formulation with Alum. The methods described to extract lipids from egg yolk and saponins from non-refined extract allowed us to obtain an inexpensive and highly effective adjuvant.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
xiaodaiduyan发布了新的文献求助10
2秒前
2秒前
艾斯喜爱完成签到,获得积分10
3秒前
小刘爱读文献完成签到 ,获得积分10
3秒前
4秒前
Lucas应助古乙丁三雨采纳,获得10
6秒前
南安完成签到 ,获得积分20
7秒前
WILD完成签到 ,获得积分10
7秒前
LZM完成签到,获得积分10
7秒前
葡萄成熟时完成签到,获得积分10
8秒前
上官若男应助出众采纳,获得10
8秒前
小狐狸完成签到,获得积分20
9秒前
善学以致用应助南宫古伦采纳,获得40
9秒前
Amber完成签到,获得积分10
9秒前
IBMffff应助科研通管家采纳,获得10
11秒前
小狐狸发布了新的文献求助10
11秒前
JamesPei应助科研通管家采纳,获得10
11秒前
NexusExplorer应助科研通管家采纳,获得10
11秒前
思源应助科研通管家采纳,获得10
11秒前
紫靛橙应助科研通管家采纳,获得10
11秒前
大个应助科研通管家采纳,获得10
11秒前
狂野萤应助科研通管家采纳,获得20
11秒前
完美世界应助科研通管家采纳,获得10
11秒前
12秒前
优雅的猪完成签到,获得积分10
14秒前
15秒前
善始善终发布了新的文献求助10
16秒前
super发布了新的文献求助10
16秒前
17秒前
18秒前
18秒前
Hello应助小狐狸采纳,获得10
18秒前
20秒前
Jasper应助欢呼的冰蝶采纳,获得10
21秒前
CodeCraft应助fifteen采纳,获得10
23秒前
赘婿应助ayan采纳,获得10
25秒前
chichenglin发布了新的文献求助10
25秒前
25秒前
26秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
2-Acetyl-1-pyrroline: an important aroma component of cooked rice 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
Handbook on People's China (1957) 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3189376
求助须知:如何正确求助?哪些是违规求助? 2838682
关于积分的说明 8021170
捐赠科研通 2501595
什么是DOI,文献DOI怎么找? 1335815
科研通“疑难数据库(出版商)”最低求助积分说明 637711
邀请新用户注册赠送积分活动 605784