Development of low-cost cage-like particles to formulate veterinary vaccines

卵清蛋白 蛋黄 明矾 佐剂 薄层色谱法 色谱法 抗体 免疫系统 生物 化学 食品科学 免疫学 有机化学
作者
Giuliana Lupi,Florencia X. Santiago Valtierra,Gabriel Cabrera,Roque Spinelli,Álvaro S. Siano,Verónica González,Antonio Osuna,Gerardo M. Oresti,Iván Marcipar
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier]
卷期号:251: 110460-110460
标识
DOI:10.1016/j.vetimm.2022.110460
摘要

Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extraction and by dialyzing a non-refined saponins extract, respectively. Lipids were fully characterized by thin layer chromatography (TLC) and gas-chromatography (GC) and enzymatic methods, and saponins were characterized by TLC, HPLC and MALDI-TOF. Cage-like particles were prepared with these components or with commercial inputs. Both particles and the traditional Alum used in veterinary vaccines were compared by immunizing mice with Ovalbumin (OVA) formulated with these adjuvants and assessing IgG1, IgG2a anti OVA antibodies and specific Delayed-type Hypersensitivity (DTH). In the yolk extract, a mixture of phospholipids, cholesterol and minor components of the extract (e.g. lyso-phospholipids) with suitable proportions to generate cage-like particles was obtained. Also, semi-purified saponins with similar features to those of the QuilA® were obtained. Cage-like particles prepared with these components have 40-50 nm and triggers similar levels of Anti-OVA IgG1 and DTH than with commercial inputs but higher specific-IgG2a. Both adjuvants largely increased the levels of IgG1, IgG2a and DTH in relation to the formulation with Alum. The methods described to extract lipids from egg yolk and saponins from non-refined extract allowed us to obtain an inexpensive and highly effective adjuvant.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
2秒前
传奇3应助毛毛虫采纳,获得10
2秒前
3秒前
3秒前
小菜鸡发布了新的文献求助80
3秒前
haapy发布了新的文献求助10
5秒前
李健应助树上香蕉果采纳,获得10
5秒前
zhangsudi发布了新的文献求助10
6秒前
优秀含烟完成签到,获得积分10
6秒前
7秒前
ferritin应助格纸哥采纳,获得10
8秒前
元不二发布了新的文献求助30
8秒前
8秒前
9秒前
干净雅旋完成签到,获得积分20
9秒前
qin希望应助空白格采纳,获得10
9秒前
10秒前
大个应助女巫Q采纳,获得10
11秒前
光亮的靖柔完成签到,获得积分10
11秒前
毛毛虫完成签到,获得积分10
15秒前
Jasper应助haapy采纳,获得10
15秒前
KKKZ完成签到,获得积分10
15秒前
田様应助认真柜子采纳,获得10
15秒前
15秒前
15秒前
zz完成签到 ,获得积分10
17秒前
宇宙大爆炸完成签到 ,获得积分10
17秒前
科研通AI2S应助明理翼采纳,获得10
17秒前
yayyaya完成签到 ,获得积分10
18秒前
18秒前
lrt_lnnu发布了新的文献求助30
18秒前
18秒前
19秒前
19秒前
冷酷石头发布了新的文献求助10
21秒前
21秒前
22秒前
大力夜雪发布了新的文献求助10
22秒前
毛毛虫发布了新的文献求助10
22秒前
23秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3182895
求助须知:如何正确求助?哪些是违规求助? 2833076
关于积分的说明 7992477
捐赠科研通 2495256
什么是DOI,文献DOI怎么找? 1331389
科研通“疑难数据库(出版商)”最低求助积分说明 636286
邀请新用户注册赠送积分活动 603350