Development of low-cost cage-like particles to formulate veterinary vaccines

卵清蛋白 蛋黄 明矾 佐剂 薄层色谱法 色谱法 抗体 免疫系统 生物 化学 食品科学 免疫学 有机化学
作者
Giuliana Lupi,Florencia X. Santiago Valtierra,Gabriel Cabrera,Roque Spinelli,Álvaro S. Siano,Verónica González,Antonio Osuna,Gerardo M. Oresti,Iván Marcipar
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier]
卷期号:251: 110460-110460
标识
DOI:10.1016/j.vetimm.2022.110460
摘要

Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extraction and by dialyzing a non-refined saponins extract, respectively. Lipids were fully characterized by thin layer chromatography (TLC) and gas-chromatography (GC) and enzymatic methods, and saponins were characterized by TLC, HPLC and MALDI-TOF. Cage-like particles were prepared with these components or with commercial inputs. Both particles and the traditional Alum used in veterinary vaccines were compared by immunizing mice with Ovalbumin (OVA) formulated with these adjuvants and assessing IgG1, IgG2a anti OVA antibodies and specific Delayed-type Hypersensitivity (DTH). In the yolk extract, a mixture of phospholipids, cholesterol and minor components of the extract (e.g. lyso-phospholipids) with suitable proportions to generate cage-like particles was obtained. Also, semi-purified saponins with similar features to those of the QuilA® were obtained. Cage-like particles prepared with these components have 40-50 nm and triggers similar levels of Anti-OVA IgG1 and DTH than with commercial inputs but higher specific-IgG2a. Both adjuvants largely increased the levels of IgG1, IgG2a and DTH in relation to the formulation with Alum. The methods described to extract lipids from egg yolk and saponins from non-refined extract allowed us to obtain an inexpensive and highly effective adjuvant.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
善学以致用应助独特大米采纳,获得10
1秒前
调研昵称发布了新的文献求助10
2秒前
冲鸭完成签到,获得积分10
2秒前
3秒前
活泼的冬寒完成签到,获得积分10
3秒前
4秒前
缓慢的盼旋完成签到 ,获得积分10
7秒前
研友_LXONx8发布了新的文献求助10
9秒前
隐形曼青应助winwin采纳,获得10
9秒前
10秒前
饼大王完成签到,获得积分10
11秒前
有魅力听枫完成签到,获得积分10
12秒前
ZYC完成签到 ,获得积分10
13秒前
芝士球球应助留胡子的霖采纳,获得10
14秒前
唔西迪西完成签到,获得积分10
15秒前
今后应助北74采纳,获得10
15秒前
16秒前
猪猪发布了新的文献求助10
17秒前
充电宝应助优秀的行云采纳,获得10
17秒前
19秒前
领导范儿应助orange采纳,获得10
20秒前
20秒前
小蚂蚁完成签到,获得积分10
20秒前
DrLin完成签到,获得积分10
21秒前
stuhwt发布了新的文献求助10
22秒前
27秒前
28秒前
爆米花应助stuhwt采纳,获得10
28秒前
iNk应助迷路荷花采纳,获得10
28秒前
串串香发布了新的文献求助20
28秒前
吴宝健完成签到,获得积分10
29秒前
31秒前
英姑应助猪猪采纳,获得10
32秒前
大刘关注了科研通微信公众号
32秒前
小王发布了新的文献求助10
32秒前
12345完成签到,获得积分10
34秒前
烟花应助怡然的雪柳采纳,获得10
34秒前
35秒前
隐形曼青应助XXH采纳,获得10
36秒前
吴宝健发布了新的文献求助10
36秒前
高分求助中
Evolution 3rd edition 1500
保险藏宝图 1000
Lire en communiste 1000
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A new species of Coccus (Homoptera: Coccoidea) from Malawi 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3182243
求助须知:如何正确求助?哪些是违规求助? 2832593
关于积分的说明 7989919
捐赠科研通 2494611
什么是DOI,文献DOI怎么找? 1330892
科研通“疑难数据库(出版商)”最低求助积分说明 636166
邀请新用户注册赠送积分活动 603070