Development of low-cost cage-like particles to formulate veterinary vaccines

卵清蛋白 蛋黄 明矾 佐剂 薄层色谱法 色谱法 抗体 免疫系统 生物 化学 食品科学 免疫学 有机化学
作者
Giuliana Lupi,Florencia X. Santiago Valtierra,Gabriel Cabrera,Roque Spinelli,Álvaro S. Siano,Verónica González,Antonio Osuna,Gerardo M. Oresti,Iván Marcipar
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier]
卷期号:251: 110460-110460
标识
DOI:10.1016/j.vetimm.2022.110460
摘要

Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extraction and by dialyzing a non-refined saponins extract, respectively. Lipids were fully characterized by thin layer chromatography (TLC) and gas-chromatography (GC) and enzymatic methods, and saponins were characterized by TLC, HPLC and MALDI-TOF. Cage-like particles were prepared with these components or with commercial inputs. Both particles and the traditional Alum used in veterinary vaccines were compared by immunizing mice with Ovalbumin (OVA) formulated with these adjuvants and assessing IgG1, IgG2a anti OVA antibodies and specific Delayed-type Hypersensitivity (DTH). In the yolk extract, a mixture of phospholipids, cholesterol and minor components of the extract (e.g. lyso-phospholipids) with suitable proportions to generate cage-like particles was obtained. Also, semi-purified saponins with similar features to those of the QuilA® were obtained. Cage-like particles prepared with these components have 40-50 nm and triggers similar levels of Anti-OVA IgG1 and DTH than with commercial inputs but higher specific-IgG2a. Both adjuvants largely increased the levels of IgG1, IgG2a and DTH in relation to the formulation with Alum. The methods described to extract lipids from egg yolk and saponins from non-refined extract allowed us to obtain an inexpensive and highly effective adjuvant.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
明理的夏岚完成签到,获得积分10
刚刚
刚刚
小军完成签到,获得积分10
3秒前
天天加油关注了科研通微信公众号
4秒前
知命的火完成签到 ,获得积分10
5秒前
Lynn发布了新的文献求助10
5秒前
是是是完成签到,获得积分10
7秒前
8秒前
8秒前
小闲鱼完成签到,获得积分10
8秒前
雨淼99发布了新的文献求助30
9秒前
10秒前
朱南晴发布了新的文献求助10
10秒前
现代雪晴完成签到,获得积分10
11秒前
13秒前
14秒前
15秒前
16秒前
嵇翩跹完成签到,获得积分10
16秒前
小陈发布了新的文献求助10
18秒前
jiegelaile完成签到,获得积分10
19秒前
21秒前
21秒前
21秒前
Aggie发布了新的文献求助10
23秒前
Downhole发布了新的文献求助10
25秒前
现代的擎苍完成签到,获得积分10
25秒前
Orange应助笑点低的满天采纳,获得10
26秒前
Jasper应助ZhongxiangDing采纳,获得10
26秒前
27秒前
李健应助artos采纳,获得10
27秒前
周周发布了新的文献求助10
27秒前
27秒前
小陈完成签到,获得积分10
28秒前
VDC应助Aggie采纳,获得30
30秒前
30秒前
30秒前
31秒前
852应助Grool采纳,获得10
32秒前
stupidZ发布了新的文献求助10
33秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
安全防范技术与工程 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
A real-time energy management strategy based on fuzzy control and ECMS for PHEVs 400
2024 Medicinal Chemistry Reviews 400
Why I Chose China [by Morris R. Wills] in "Look", February 8 and 22, 1966; 400
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3191484
求助须知:如何正确求助?哪些是违规求助? 2840825
关于积分的说明 8030243
捐赠科研通 2504195
什么是DOI,文献DOI怎么找? 1337556
科研通“疑难数据库(出版商)”最低求助积分说明 638102
邀请新用户注册赠送积分活动 606622