Development of low-cost cage-like particles to formulate veterinary vaccines

卵清蛋白 蛋黄 明矾 佐剂 薄层色谱法 色谱法 抗体 免疫系统 生物 化学 食品科学 免疫学 有机化学
作者
Giuliana Lupi,Florencia X. Santiago Valtierra,Gabriel Cabrera,Roque Spinelli,Álvaro S. Siano,Verónica González,Antonio Osuna,Gerardo M. Oresti,Iván Marcipar
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier]
卷期号:251: 110460-110460
标识
DOI:10.1016/j.vetimm.2022.110460
摘要

Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extraction and by dialyzing a non-refined saponins extract, respectively. Lipids were fully characterized by thin layer chromatography (TLC) and gas-chromatography (GC) and enzymatic methods, and saponins were characterized by TLC, HPLC and MALDI-TOF. Cage-like particles were prepared with these components or with commercial inputs. Both particles and the traditional Alum used in veterinary vaccines were compared by immunizing mice with Ovalbumin (OVA) formulated with these adjuvants and assessing IgG1, IgG2a anti OVA antibodies and specific Delayed-type Hypersensitivity (DTH). In the yolk extract, a mixture of phospholipids, cholesterol and minor components of the extract (e.g. lyso-phospholipids) with suitable proportions to generate cage-like particles was obtained. Also, semi-purified saponins with similar features to those of the QuilA® were obtained. Cage-like particles prepared with these components have 40-50 nm and triggers similar levels of Anti-OVA IgG1 and DTH than with commercial inputs but higher specific-IgG2a. Both adjuvants largely increased the levels of IgG1, IgG2a and DTH in relation to the formulation with Alum. The methods described to extract lipids from egg yolk and saponins from non-refined extract allowed us to obtain an inexpensive and highly effective adjuvant.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
lll完成签到 ,获得积分10
1秒前
2秒前
3秒前
一定要早睡完成签到,获得积分10
4秒前
研究牲完成签到,获得积分10
5秒前
小郭完成签到,获得积分10
5秒前
6秒前
8R60d8应助一个橡果采纳,获得10
6秒前
7秒前
培培完成签到 ,获得积分10
7秒前
哈哈哈哈完成签到 ,获得积分10
8秒前
8秒前
鱼鱼片片发布了新的文献求助10
9秒前
9秒前
LYL发布了新的文献求助10
10秒前
10秒前
11秒前
11秒前
er发布了新的文献求助10
11秒前
斯文败类应助学不懂采纳,获得30
12秒前
穿裤子的发糕完成签到,获得积分20
12秒前
毛豆应助小郭采纳,获得10
13秒前
14秒前
Ling发布了新的文献求助10
14秒前
15秒前
Rita发布了新的文献求助10
15秒前
1234发布了新的文献求助10
15秒前
丘比特应助阔达的棒棒糖采纳,获得10
16秒前
16秒前
充电宝应助淡然的鸿煊采纳,获得10
17秒前
小圆发布了新的文献求助10
17秒前
18秒前
19秒前
juanjuan应助Aijh采纳,获得10
19秒前
自由念之完成签到,获得积分10
21秒前
yexiao发布了新的文献求助10
21秒前
振耳欲聋的沉默完成签到,获得积分10
22秒前
清爽的秀发布了新的文献求助10
23秒前
23秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Write Like a Chemist: A Guide and Resource (第二版) 600
Mixed-anion Compounds 600
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
Earth System Geophysics 500
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Geochemistry, 2nd Edition 地球化学经典教科书第二版 401
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3199568
求助须知:如何正确求助?哪些是违规求助? 2848333
关于积分的说明 8067643
捐赠科研通 2513090
什么是DOI,文献DOI怎么找? 1345419
科研通“疑难数据库(出版商)”最低求助积分说明 640058
邀请新用户注册赠送积分活动 609751