Development of low-cost cage-like particles to formulate veterinary vaccines

卵清蛋白 蛋黄 明矾 佐剂 薄层色谱法 色谱法 抗体 免疫系统 生物 化学 食品科学 免疫学 有机化学
作者
Giuliana Lupi,Florencia X. Santiago Valtierra,Gabriel Cabrera,Roque Spinelli,Álvaro S. Siano,Verónica González,Antonio Osuna,Gerardo M. Oresti,Iván Marcipar
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier]
卷期号:251: 110460-110460
标识
DOI:10.1016/j.vetimm.2022.110460
摘要

Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extraction and by dialyzing a non-refined saponins extract, respectively. Lipids were fully characterized by thin layer chromatography (TLC) and gas-chromatography (GC) and enzymatic methods, and saponins were characterized by TLC, HPLC and MALDI-TOF. Cage-like particles were prepared with these components or with commercial inputs. Both particles and the traditional Alum used in veterinary vaccines were compared by immunizing mice with Ovalbumin (OVA) formulated with these adjuvants and assessing IgG1, IgG2a anti OVA antibodies and specific Delayed-type Hypersensitivity (DTH). In the yolk extract, a mixture of phospholipids, cholesterol and minor components of the extract (e.g. lyso-phospholipids) with suitable proportions to generate cage-like particles was obtained. Also, semi-purified saponins with similar features to those of the QuilA® were obtained. Cage-like particles prepared with these components have 40-50 nm and triggers similar levels of Anti-OVA IgG1 and DTH than with commercial inputs but higher specific-IgG2a. Both adjuvants largely increased the levels of IgG1, IgG2a and DTH in relation to the formulation with Alum. The methods described to extract lipids from egg yolk and saponins from non-refined extract allowed us to obtain an inexpensive and highly effective adjuvant.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
刚刚
wang完成签到,获得积分10
刚刚
刚刚
Nice2Cu完成签到,获得积分10
2秒前
希望天下0贩的0应助劳模采纳,获得10
2秒前
3秒前
NexusExplorer应助民科王聪采纳,获得10
3秒前
3秒前
清新的帅哥完成签到,获得积分10
4秒前
4秒前
4秒前
4秒前
4秒前
唐瑞玖完成签到,获得积分10
5秒前
Owen应助和谐的孱采纳,获得10
5秒前
6秒前
不配.应助tlhust采纳,获得10
7秒前
7秒前
必中发布了新的文献求助10
8秒前
8秒前
9秒前
学术狗发布了新的文献求助10
9秒前
Alan发布了新的文献求助10
9秒前
10秒前
声声慢完成签到,获得积分10
10秒前
852应助袁华采纳,获得10
10秒前
临风发布了新的文献求助10
12秒前
13秒前
13秒前
星辰大海应助ccccc采纳,获得10
14秒前
eureka发布了新的文献求助10
15秒前
17秒前
学术狗完成签到,获得积分10
17秒前
Alan完成签到,获得积分10
17秒前
田様应助IKUN采纳,获得10
17秒前
QQQQ完成签到,获得积分20
17秒前
然大宝发布了新的文献求助10
18秒前
夏惋清完成签到 ,获得积分0
19秒前
19秒前
20秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
Aspects of Babylonian celestial divination: the lunar eclipse tablets of Enūma Anu Enlil 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
India's foreign trade policy and its performance in the world economy 450
Structural Inorganic Chemistry 400
Dictionary of socialism 350
Mixed-anion Compounds 300
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3196121
求助须知:如何正确求助?哪些是违规求助? 2844892
关于积分的说明 8052117
捐赠科研通 2509514
什么是DOI,文献DOI怎么找? 1341768
科研通“疑难数据库(出版商)”最低求助积分说明 639262
邀请新用户注册赠送积分活动 608445