Development of low-cost cage-like particles to formulate veterinary vaccines

卵清蛋白 蛋黄 明矾 佐剂 薄层色谱法 色谱法 抗体 免疫系统 生物 化学 食品科学 免疫学 有机化学
作者
Giuliana Lupi,Florencia X. Santiago Valtierra,Gabriel Cabrera,Roque Spinelli,Álvaro S. Siano,Verónica González,Antonio Osuna,Gerardo M. Oresti,Iván Marcipar
出处
期刊:Veterinary Immunology and Immunopathology [Elsevier]
卷期号:251: 110460-110460
标识
DOI:10.1016/j.vetimm.2022.110460
摘要

Low-cost adjuvants are urgently needed for the development of veterinary vaccines able to trigger strong immune responses. In this work, we describe a method to obtain a low-cost cage-like particles (ISCOMATRIX-like) adjuvant useful to formulate veterinary vaccines candidates. The main components to form the particles are lipids and saponins, which were obtained from egg yolk by ethanolic extraction and by dialyzing a non-refined saponins extract, respectively. Lipids were fully characterized by thin layer chromatography (TLC) and gas-chromatography (GC) and enzymatic methods, and saponins were characterized by TLC, HPLC and MALDI-TOF. Cage-like particles were prepared with these components or with commercial inputs. Both particles and the traditional Alum used in veterinary vaccines were compared by immunizing mice with Ovalbumin (OVA) formulated with these adjuvants and assessing IgG1, IgG2a anti OVA antibodies and specific Delayed-type Hypersensitivity (DTH). In the yolk extract, a mixture of phospholipids, cholesterol and minor components of the extract (e.g. lyso-phospholipids) with suitable proportions to generate cage-like particles was obtained. Also, semi-purified saponins with similar features to those of the QuilA® were obtained. Cage-like particles prepared with these components have 40-50 nm and triggers similar levels of Anti-OVA IgG1 and DTH than with commercial inputs but higher specific-IgG2a. Both adjuvants largely increased the levels of IgG1, IgG2a and DTH in relation to the formulation with Alum. The methods described to extract lipids from egg yolk and saponins from non-refined extract allowed us to obtain an inexpensive and highly effective adjuvant.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
OeO完成签到 ,获得积分10
刚刚
坦率的匪完成签到,获得积分10
刚刚
小硕土川发布了新的文献求助30
刚刚
chu完成签到,获得积分10
2秒前
Hello应助syrrr要发文章采纳,获得10
2秒前
啦啦啦完成签到,获得积分10
3秒前
王胖完成签到 ,获得积分10
3秒前
幸福的千琴完成签到,获得积分10
3秒前
3秒前
科研通AI2S应助cgmil采纳,获得10
4秒前
华仔应助gdh采纳,获得10
4秒前
Jasper应助xigua采纳,获得30
4秒前
希望天下0贩的0应助Yulin Yu采纳,获得10
4秒前
西柚完成签到,获得积分10
5秒前
坦率雁卉完成签到,获得积分10
5秒前
gxpjzbg完成签到,获得积分10
6秒前
小乔应助ATREE采纳,获得10
6秒前
YuLu完成签到 ,获得积分10
6秒前
我要发十篇sci完成签到 ,获得积分10
7秒前
兴奋元灵完成签到 ,获得积分10
8秒前
bkagyin应助马尼拉采纳,获得10
8秒前
9秒前
cham完成签到,获得积分10
9秒前
9秒前
小蒋完成签到,获得积分20
10秒前
10秒前
PFD000完成签到,获得积分10
10秒前
科研通AI2S应助sunshine采纳,获得10
10秒前
啦啦啦完成签到,获得积分20
10秒前
11秒前
11秒前
baby发布了新的文献求助10
12秒前
12秒前
顾晓完成签到,获得积分10
12秒前
优雅的怀莲完成签到,获得积分10
12秒前
13秒前
陌君子筱完成签到,获得积分10
13秒前
chloe完成签到,获得积分10
13秒前
xmy完成签到,获得积分10
14秒前
xjjw发布了新的文献求助10
15秒前
高分求助中
Spray / Wall-interaction Modelling by Dimensionless Data Analysis 2000
ALA生合成不全マウスでの糖代謝異常の分子機構解析 520
安全防范技术与工程 500
Mathematics and Finite Element Discretizations of Incompressible Navier—Stokes Flows 500
2024 Medicinal Chemistry Reviews 400
Актуализированная стратиграфическая схема триасовых отложений Прикаспийского региона. Объяснительная записка 360
Dictionary of socialism 350
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3192935
求助须知:如何正确求助?哪些是违规求助? 2841978
关于积分的说明 8036528
捐赠科研通 2505759
什么是DOI,文献DOI怎么找? 1338749
科研通“疑难数据库(出版商)”最低求助积分说明 638486
邀请新用户注册赠送积分活动 606990