Rational Design of Specific Recognition Molecules for Simultaneously Monitoring of Endogenous Polysulfide and Hydrogen Sulfide in the Mouse Brain

Abstract A biosensor was created for the simultaneous monitoring of endogenous H 2 S n and H 2 S in mouse brains and exploring their roles in activation of the TRPA1 channel under two types of brain disease models: ischemia and Alzheimer's disease (AD). Based on DFT calculations and electrochemical measurements, two probes, 3,4‐bis((2‐fluoro‐5‐nitrobenzoyl)oxy)‐benzoic acid (M PS‐1 ) and N‐(4‐(2,5‐dinitrophenoxy) phenyl)‐5‐(1, 2‐dithiolan‐3‐yl)pentanamide (M HS‐1 ), were synthesized for specific recognition of H 2 S n and H 2 S. Through co‐assembly of the two probes at the mesoporous gold film with good anti‐biofouling ability and electrocatalytic activity, this microsensor showed high selectivity for H 2 S n and H 2 S against potential biological interferences. The biosensor can simultaneously determine the concentration of H 2 S n from 0.2 to 50 μ m , as well as that of H 2 S from 0.2 to 40 μ m . The expression of TRPA1 protein positively correlated with levels of H 2 S n under both ischemia and AD.