光热治疗
库尔特计数器
化学
微流控
电流(流体)
纳米技术
离子流
光热效应
流式细胞术
光热光谱学
离子
光电子学
材料科学
遗传学
工程类
分子生物学
有机化学
电气工程
生物
作者
Chang Heon Lee,Ji Tae Kim,Dong-won Jeong,Sang Hyun Lee,Yoonhee Kim,Seok Hee Han,Myeongsik Shin,Taek Dong Chung
标识
DOI:10.1021/acs.analchem.4c02381
摘要
Blood cell counting typically requires complex machinery. Flow cytometers used for this purpose involve precise optical alignment, costly detectors, and pretreatment with fluorescent labels. Coulter countertype devices, which monitor ion current, are simpler. However, conventional Coulter counters provide only information about size, making it impossible to distinguish similarly sized lymphocytes from red blood cells (RBCs). Inspired by the fact that RBCs have an exceptionally high propensity to absorb light and convert it to heat, i.e., photothermal effect, this study proposes integrating photothermal phenomena into a microfluidic Coulter counting chip. Photothermal heat selectively amplifies the ion current from RBCs over other components including lymphocytes. The combination of ion current monitoring and the photothermal effect for RBC counting suggests an evolution toward versatile flow cytometers.
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