医学
高氧
自噬
机制(生物学)
内皮干细胞
细胞损伤
细胞生物学
肺
内科学
细胞凋亡
生物化学
生物
认识论
哲学
化学
体外
作者
Xiaodan Zhu,Shasha He,Rong Zhang,Lan Kang,Xiaoping Lei,Wenbin Dong
出处
期刊:American Journal of Perinatology
[Georg Thieme Verlag KG]
日期:2023-07-29
卷期号:41 (S 01): e2365-e2375
被引量:1
标识
DOI:10.1055/s-0043-1771258
摘要
Objective Bronchopulmonary dysplasia is a chronic lung disease in premature infants with alveolar simplification and pulmonary vascular development disorder as the main pathological feature and hyperoxia as the main etiology. Autophagy is a highly conserved cytological behavior of self-degrading cellular components and is accompanied by oxidative stress. Studies have reported that autophagy is regulated by FOXO1 posttranslational modification. However, whether autophagy can be involved in the regulation of endothelial cell injury induced by hyperoxia and its mechanism are still unclear. Study Design We have activated and inhibited autophagy in human umbilical vein endothelial cells under hyperoxia and verified the role of autophagy in endothelial cell-related functions from both positive and negative aspects. Results Our research showed that the expression level of autophagy-related proteins decreased, accompanied by decreased cell migration ability and tube formation ability and increased cell reactive oxygen species level and cell permeability under hyperoxia conditions. Using an autophagy agonist alleviated hyperoxia-induced changes and played a protective role. However, inhibition of autophagy aggravated the cell damage induced by hyperoxia. Moreover, the decrease in autophagy proteins was accompanied by the upregulation of FOXO1 phosphorylation and acetylation. Conclusion We concluded that autophagy was a protective mechanism against endothelial cell injury caused by hyperoxia. Autophagy might participate in this process by coregulating posttranslational modifications of FOXO1. Key Points
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