低温保护剂
低温保存
生物
低温生物学
胚泡
水通道蛋白3
甘油
二甲基亚砜
水运
乙二醇
膜透性
男科
水通道蛋白
胚胎
细胞生物学
生物化学
化学
胚胎发生
膜
水流
有机化学
工程类
环境工程
医学
作者
Bo Jin,Ryu-ichi Higashiyama,Yu-ichi Nakata,Jun-ichi Yonezawa,Shangdan Xu,Masashi Miyake,Seiichi Takahashi,Kazuhiro Kikuchi,Ken-ichi Yazawa,Shuhei Mizobuchi,Saori Niimi,Mizuho Kitayama,Chihiro Koshimoto,Kazutsugu Matsukawa,Magosaburo Kasai,Keisuke Edashige
标识
DOI:10.1095/biolreprod.112.107250
摘要
Pig oocytes and embryos are highly sensitive to cryopreservation; however, tolerance to cryopreservation increases in embryos at the expanded blastocyst stage. This increased tolerance may be attributed to a decrease in cytoplasmic lipid droplets at this stage. We previously showed that an increase in the permeability of the plasma membrane in mouse oocytes to water and cryoprotectants, caused by the artificial expression of aquaporin 3, an aquaglyceroporin, enhanced tolerance to cryopreservation. In the present study, we investigated whether membrane permeability was also involved in the tolerance of pig embryos to cryopreservation. The permeability of oocytes and morulae to water and glycerol was low, whereas that of expanded blastocysts was high. Activation energy for permeability to water, glycerol, ethylene glycol, and dimethyl sulfoxide was markedly lower for expanded blastocysts than for oocytes. This suggests that water and these cryoprotectants move through expanded blastocysts predominantly by facilitated diffusion and through oocytes predominantly by simple diffusion. Aquaporin 3 mRNA was expressed in expanded blastocysts abundantly, but less so in oocytes. On the other hand, the permeability of expanded blastocysts to propylene glycol was as low as that of oocytes, and activation energy for its permeability was similar to that of oocytes, which suggests that propylene glycol moves through oocytes and embryos predominantly by simple diffusion. These results suggest that the higher tolerance of pig expanded blastocysts to cryopreservation is also related to high membrane permeability due to the expression of water/cryoprotectant channels, in addition to the decrease in cytoplasmic lipid droplets.
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